Sequence analysis of mitochondrial <I>COI</I> gene fragment of six Veneridae clams(Mollusca:Bivalvia) and four populations of <I>Meretrix meretrix</I>

CHENG Han-liang; XIA De-quan; WU Ting-ting; MENG Xue-ping; JI Hong-jiu; DONG Zhi-guo; CHEN Shu-yin

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CHENG Han-liang, XIA De-quan, WU Ting-ting, MENG Xue-ping, JI Hong-jiu, DONG Zhi-guo, CHEN Shu-yin. Sequence analysis of mitochondrial COI gene fragment of six Veneridae clams(Mollusca:Bivalvia) and four populations of Meretrix meretrix[J]. Haiyang Xuebao, 2007, 29(5): 109-116.

Citation:

CHENG Han-liang, XIA De-quan, WU Ting-ting, MENG Xue-ping, JI Hong-jiu, DONG Zhi-guo, CHEN Shu-yin. Sequence analysis of mitochondrial COI gene fragment of six Veneridae clams(Mollusca:Bivalvia) and four populations of Meretrix meretrix[J]. Haiyang Xuebao, 2007, 29(5): 109-116.

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Sequence analysis of mitochondrial COI gene fragment of six Veneridae clams(Mollusca:Bivalvia) and four populations of Meretrix meretrix

1.
Huaihai Institute of Technology, Jiangsu Key Laboratory of Marine Biotechnology, Lianyungang 222005, China;Fisheries College, Nanjing Agriculture University, Wuxi 214081, China
2.
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
3.
Fisheries College, Nanjing Agriculture University, Wuxi 214081, China
4.
Jiangsu Institute of Marine Fisheries, Nantong 226007, China

Received Date: 2006-02-23
Rev Recd Date: 2006-10-14

Abstract

Abstract

A nucleotide sequence analysis of mitochondrial cytochrome coxidase subunit I (COI) gene fragment was conducted among six family Vener idae clams, Meretrix meretrix L., 1758, Cyclina sinensis G., 1791, Mercenaria mercenaria L., 1758, Protothaca jedoensis L., 1874, Dosinia corrugata R., 1850, Ruditapes philippinarum A., 1850 and four populations of M.meretrix to determine the utility of this regions in identifying phylogeog raphic and phylog enetic structure.Nucleotide sequences of 709 bp in length were obtained from the mitochondrial DNA cytochrome coxidase subunit I (COI) gene for 18 Veneridae specimens representing six species and four geographical populations of M.meretrix, with A+T contents from 62.2% to 67.6%, which higher than G+C contents obviously.Analysis of sequence variation at the COI gene across six species revealed 311 variable sites, which 202 were par sim-informative sites.Translation of codons into amino acids indicated 85 varable sites.Analysis of sequence variation across four M.meretrix populations revealed 46 variable sites, which only two were parsim-informative sites.Translation of codons into amino acids indicated only one variable site.The phylogenetic tree of Veneridae clams was reconstructed by using COI gene sequences as marker, and with Solen grandis as outg roups.Tree topologies indicated that four M.meretrix populations formed a monophy letic group with its sister taxon C.sinensis, all Veneridae species form a clade, which was consistent with the tree based on morphological characters.This result reveals that the COI gene was an appropriate marker for studies of phylogeny and phylogeography of the Veneridae clams.
- Veneridae,
- mitochondrial DNA,
- cytochrome c oxidase subunit I,
- phylogeny,
- phylogeography

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References(27)

References

庄启谦.中国动物志-软体动物门:双壳纲:帘蛤科[M].北京:科学出版社,2001.1-10.

CANAPA A,SCHIAPARELLI S,MAROTA I,et al.Molecular data from the 16S rRNA gene for the phylogeny of Veneridae (Mollusca:Bivalvia)[J].Marine Biology,2003,142 (6):1 125-1 130.

MAYNARD B T,KERR L J,MCKIERNAN J M,et al.Mitochnodrial DNA sequence and gene organization in the Australian blacklip abalone Haliotis rubra (Leach)[J].Mar Biotechnol,2005,7:645-658.

MILBURY C A,GAFFNEY P M.Complete Mitochondrial DNA Sequence of the Eastern Oyster Crassostrea virginica[J].Mar Biotechnol,2005,7:697-712.

PASSAMONTI M,BOORE J L,SCALI V.Molecular evolution and recombination in gender-associated mitochondrial DNAs of the Manila clam Tapes philippinarun[J].Genetics,2003,164:603-611.

陈丽梅,孔晓瑜,喻子牛,等.3种蛏类线粒体16S rRNA和COI基因片段的序列比较及其系统学初步研究[J].海洋科学,2005,29(8):27-32.

PARK J K,KIM W.Two Corbicula (Corbiculidae:Bivalvia) mitochondrial lineages are widely distributed in Asian freshwater environment[J].Molecular Phylogenetics and Evolution,2003,29:529-539.

PFENNINGER K,REINHARDT F,STREIT B.Evidence for cryptic hybridization between different evolutionary lineages of the invasive clam genus Corbicula (Veneroida,Bivalvia)[J].J Evol Biol,2002,15:818-829.

SMITH P J,MCVEAGH S M,Won Y,et al.Genetic heterogeneity among New Zealand species of hydrothermal vent mussels (Mytilidae:Bathymodiolus)[J].Marine Biology,2004,144:537-545.

THERRIAULT T W,DOCKER M F,ORLOVA M I,et al.Molecular resolution of the family Dreissenidae (Mollusca:Bivalvia) with emphasis on Ponto-Caspian species,including first report of Mytilopsis leucophaeata in the Black Sea basin[J].Molecular Phylogenetics and Evolution,2004,30:479-489.

KING T L,EACKLES M S,GJETVAJ B,et al.Intraspecific phylogeography of Lasmigona subviridis (Bivalvia:Unionidae):conservation implications of range discontinuity[J].Molecular Ecology,1999,8:S65-S78.

NAGASHIMA K,SATO M,KAWAMATA K,et al.Genetic structure of Japanese scallop population in Hokkaido,analyzed by mitochondrial haplotype distribution[J].Mar Biotechnol,2005,7:1-10.

STEPIEN C A,MORTON B,DABROWSKA K A,et al.Genetic diversity and evolutionary relationships of the troglodytic living fossil Congeria kusceri (Bivlvia:Dreissenidae)[J].Molecular Ecology,2001,10:1 873-1 879.

AN H S,JEE Y J,MINK S,et al.Phylogenetic analysis of six species of pacific abalone (Haliotidae) based on DNA sequences of 16S rRNA and cytochrome c oxidase subunit Ⅰ mitochondrial genes[J].Mar Biotechnol,2005,7:373-380.

CARLINI D B,YOUNG R E,VECCHIONE M.A molecular phylogeny of the Octopoda (Mollusca:Cephalopods) evaluated in light of Morphological evidence[J].Molecular Phylogenetics and Evolution,2001,21(3):388-397.

DONALD K M,KENNEDY M,SPENCER H G.The phylogeny and taxonomy of austral monodontine topshells (Mollusca:Gastropoda:Trochidae),inferred from DNA sequences[J].Molecular Phylogenetics and Evolution,2005,37:474-483.

GOFFREDI S K,HURTADO L A,HALLAM S,et al.Evolutionary relationships of deep-sea vent and cold seep clams (Mollusca:Vesicomyidae) of the "paciffca/lepta" species complex[J].Marine Biology,2003,142:311-320.

LIN Xiang-zhi,ZHENG Xiao-dong,XIAO Shu,et al.Phylogeny of the cuttlefishes (Mollusca:Cephalopoda) based on mitochondrial COI and 16S rRNA gene sequence data[J].Acta Oceanologica Sinica,2004,23(4):699-707.

MACHORDOM A,ARAUJO R,ERPENBECK D,et al.Phylogeography and conservation genetics of endangered European Margaritiferidae (Bivalvia:Unionoidea)[J].Biological J of the Linnean Societh,2003,78:235-252.

MINTON R L,LYDEARD C.Phylogeny,taxonomy,genetics and global heritage ranks of an imperiled freshwater snail genus Lithasia (pleuroceridae)[J].Molecular Ecology,2003,12:75-87.

NIKULA R and V IN L R.Phylogeography of Cerastoderma glaucum (Bivlvia:Cardiidae) across Europe:a major break in the Eastern Mediterranean[J].Marine Biology,2003,143:339-350.

WINNEPENNINCKX B,BACKELJAU T,De WACHTER R.Extraction of high molecular weight DNA from mollusks[J].Trends Genet,1993,9:407.

FOLMER O,BLACK M,HOEH W,et al.DNA primers for amplification of mitochondrial cytochrome c oxidase subunit Ⅰ from diverce metazoan invertebrates[J].Mol Mar Biol Biotech,1994,3:294-299.

KUMAR S,TAMURA K,NEI M.MEGA3:Integrated software for molecular evolutionary genetics analysis and sequence alignment[J].Briefings in Bioinformatics,2004,5:150-163.

郭天慧,孔晓瑜,陈四清,等.三疣梭子蟹线粒体DNA 16S rRNA和COI基因片段序列的比较研究[J].中国海洋大学学报,2004,34(1):22-28.

孔晓瑜,喻子牛,刘亚军,等.中华绒螯蟹与日本绒螯蟹线粒体COI基因片段的序列比较研究[J].青岛海洋大学学报,2001,31(6):861-866.

林元烧,曹文清,方旅平,等.中华假磷虾线粒体DNA COI基因片段序列分析[J].厦门大学学报(自然科学版),2004,43(6):842-846.

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