Starvation responses of pathogenic <i>Vibrio parahaemolyticus</i> in natural Seawater

YAN Qing-pi; YI Jia-bo; CHEN Qiang; ZOU Wenzheng; ZHUANG Zhi-xia; WANG Xiao-ru

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Article Navigation > Haiyang Xuebao > 2007 > 29(3): 131-136

YAN Qing-pi, YI Jia-bo, CHEN Qiang, ZOU Wenzheng, ZHUANG Zhi-xia, WANG Xiao-ru. Starvation responses of pathogenic Vibrio parahaemolyticus in natural Seawater[J]. Haiyang Xuebao, 2007, 29(3): 131-136.

Citation:

YAN Qing-pi, YI Jia-bo, CHEN Qiang, ZOU Wenzheng, ZHUANG Zhi-xia, WANG Xiao-ru. Starvation responses of pathogenic Vibrio parahaemolyticus in natural Seawater[J]. Haiyang Xuebao, 2007, 29(3): 131-136.

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Starvation responses of pathogenic Vibrio parahaemolyticus in natural Seawater

1.
Fisheries College of Jimei University, Xiamen 361021, China
2.
Fisheries College, Ocean University of China, Qingdao 266003, China
3.
College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China

Received Date: 2006-01-10
Rev Recd Date: 2006-06-30

Abstract

Abstract

Pathogenic V.parahaemolyticus cells in log-phase were collected and the concentration was adjusted to 10⁷ cells/cm³ in natural sea water for starvation studies.The results showed that: total bacteria number、viable bacteria number and CFU number of V.parahaemolyticus increased remarkably at the initial starvation stages;total bacteria number、viable bacteria number and CFU number fell at metaphase and anaphase,among them,the total bacteria number and viable bacteria number fell slowly while the CFU number fell more quickly.Before starvation,the V.parahaemolyticus cells were shortrods and dyed equably;after starvation,a light area appeared at the cell centre and some cells turned into oval.The amount of V.parahaemolyticus cells adhered to the large yellow croaker epidermal mucus dropped sharply during starvation,approaching the level of the control after 7 days starvation.The cellular protein of V.parahaemolyticus was extracted and analyzed by SDS-PAGE,the results showed that: bacteria starved for 30 d had less protein bands in the gel than the unstarved ones;bacteria starved for 60 d had more protein bands compared to those starved for 30 d,but less than the unstarved ones.The results obtained were useful to the underst anding of the epidemiologic characteristics of V.parahaemolyticus.
- Vibrio parahaemolyticus,
- starvation,
- survival,
- adhesion

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References(25)

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