Molecular cloning and characterization of a homologue of defender against cell death 1 in <i>Epinephelus awoara</i>

WANG Li; WU Xin-zhong

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WANG Li, WU Xin-zhong. Molecular cloning and characterization of a homologue of defender against cell death 1 in Epinephelus awoara[J]. Haiyang Xuebao, 2007, 29(3): 125-130.

Citation:

WANG Li, WU Xin-zhong. Molecular cloning and characterization of a homologue of defender against cell death 1 in Epinephelus awoara[J]. Haiyang Xuebao, 2007, 29(3): 125-130.

WANG Li, WU Xin-zhong. Molecular cloning and characterization of a homologue of defender against cell death 1 in Epinephelus awoara[J]. Haiyang Xuebao, 2007, 29(3): 125-130.

Citation:

WANG Li, WU Xin-zhong. Molecular cloning and characterization of a homologue of defender against cell death 1 in Epinephelus awoara[J]. Haiyang Xuebao, 2007, 29(3): 125-130.

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Molecular cloning and characterization of a homologue of defender against cell death 1 in Epinephelus awoara

College of Animal Sciences, Zhejiang University, Hangzhou 310029, China

Received Date: 2006-07-17
Rev Recd Date: 2007-01-12

Abstract

Abstract

Defender against cell death 1(DAD1) is a negative regulator of apoptosis and a subunit of the oligosaccharyltransferase complex.We cloned DAD1 from the spleen of Epinephelus awoara using the combinative methods of suppressive subtraction hybridization,SMART cDNA sy nthesis and RACE-PCR.The full-length cDNA of DAD1 is 721 bp,containing a 342 bp open reading frame that encodes a 113 ami no acid protein.Semi-quantitative RT-PCR analyses showed that mRNA of DAD1 can be detected in the spleen,heart,liver,anterior kidney and kidney of normal E.awoara and lipopolysaccharide-stimulated E.awoara.The expressions of DAD1 in all these tissues showed some increase in the LPS-treated E.awoara,compared with normal E.awoara,which indicates that lipopolysaccharide might be a stimulator of programmed cell death.
- defender against cell death 1,
- cloning,
- expression,
- Epinephelus awoara

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References(25)

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