Artificial induction of gynogenesis in silver sea bream(<i>Rhabdosargus sarba</i>)

CAI Ze-ping; HU Chao-qun; NIU Wen-tao; ZHANG Jun-bin

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CAI Ze-ping, HU Chao-qun, NIU Wen-tao, ZHANG Jun-bin. Artificial induction of gynogenesis in silver sea bream(Rhabdosargus sarba)[J]. Haiyang Xuebao, 2007, 29(3): 114-119.

Citation:

CAI Ze-ping, HU Chao-qun, NIU Wen-tao, ZHANG Jun-bin. Artificial induction of gynogenesis in silver sea bream(Rhabdosargus sarba)[J]. Haiyang Xuebao, 2007, 29(3): 114-119.

CAI Ze-ping, HU Chao-qun, NIU Wen-tao, ZHANG Jun-bin. Artificial induction of gynogenesis in silver sea bream(Rhabdosargus sarba)[J]. Haiyang Xuebao, 2007, 29(3): 114-119.

Citation:

CAI Ze-ping, HU Chao-qun, NIU Wen-tao, ZHANG Jun-bin. Artificial induction of gynogenesis in silver sea bream(Rhabdosargus sarba)[J]. Haiyang Xuebao, 2007, 29(3): 114-119.

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Artificial induction of gynogenesis in silver sea bream(Rhabdosargus sarba)

South China Sea Institute of Oceanology, Chinese Academy of Sciences;LAMB of Guangdong Province and the Chinese Academy of Sciences, Guangzhou 510301, China

Received Date: 2005-10-09
Rev Recd Date: 2006-03-30

Abstract

Abstract

The silver sea bream(Rhabdosar gussarba) is a valuable fishery and aquaculture species in Asia,with sexually dimorphic growth in favor of the females.Gynogenetic diploids were induced using UV-irradiated sperm of conspecifics and cold shock.In the experiments,the sperm was diluted in 1:60 in disinfected natural seawater and then irradiated for 100 sata UV intensity of 700 L W/(cm²·s).This irradiation duration inactivated the spermatozoagenetically but still maintained their physiological vitality to activate eggs.95%~100% of haploid rate was obtained when non cold shock was used.Suitable conditions of time to initiate cold shock,cold shock tem perature and duration to restore the diploidy were evaluated.The results showed the fertilization rate and hatching rate of gynogenetic diploid were 71.3%~82.7% and 24.7%~36.3%,respectively by applying cold shock at 0~8℃ to eggs activated with UV-irradiated homologous sperm,5 min after activation,with shock duration of 15min.The optimum cold shock temperature was 2~6℃.60.0%~67.1% fertilization rate and 30.6%~36.5% hatching rate was attained starting cold shock 4~6 min after fertilization,cold shock at 3℃ for 15 min.Cold shock duration of 11~19 min was appro priate in inducing gynogenesis,which resulted in 46.9%~65.8% fertilization rate and 31.7%~42.4% hatching rate.The results of experiments indicated that eggs were exposed to a cold shock at 3℃,5 min after activation with shock duration of 15min was significantly effective for induction of diploid gynogenesis in silver sea bream.Mass production of diploid gynogenetic fry is possible in this prolific species.
- silver sea bream (Rhabdosargus sarba),
- cold shock,
- gynogenesis,
- artificial induction,
- UV-irradiation,

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References(22)

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