Morphological and RAPD variation among five polulations of Coelomactra antiquata

The Randomly Amplified Polymorphic DNA(RAPD) technique was applied to assess the genetic variation among five populations of Coelomactra antiquata.Under predetermined optimal reaction conditions,168 RAPD sites were detected ranging from 250 bp to 3 000 bp with 20 primers.The number of amplified bands ranged from 3 t o 15 in every individual.The experimental data were analyzed with the Popgen1.32 and PHYLIP software.The results showed that the genetic distance among individuals of one population and between different polulations were high.the morphological variation and genetic distance of Beihai population were different from other four populations,we presumed it have been a geographical population.The mean heterozygosities and mean proportion of polymorphic loci of five population were 78.97% and 0.308 7 respectively,value of Shannondiversity ranging from 0.301 8 to 0.367 3;the nearest phylogenetic relationship occure between the Shandong and Jiangsu populations,then between the Zhejiang and Fujian groups in turn.It's shown that the resource of Coelomactra antiquata was in good condition with high genetic variation and the prospect of Coelomactraantiquata culture was good.