Study on the sensitivities of eight antibiotics on <i>Alexandrium tamarense (Lebour) Balech</i>

ZHANG Dong-bao; SUI Zheng-hong; MAO Yun-xiang; GUO Hao; ZANG Xiao-nan; ZHANG Xue-cheng; LU YING-gang

Article Contents

Article Navigation > Haiyang Xuebao > 2007 > 29(2): 123-130

ZHANG Dong-bao, SUI Zheng-hong, MAO Yun-xiang, GUO Hao, ZANG Xiao-nan, ZHANG Xue-cheng, LU YING-gang. Study on the sensitivities of eight antibiotics on Alexandrium tamarense (Lebour) Balech[J]. Haiyang Xuebao, 2007, 29(2): 123-130.

Citation:

ZHANG Dong-bao, SUI Zheng-hong, MAO Yun-xiang, GUO Hao, ZANG Xiao-nan, ZHANG Xue-cheng, LU YING-gang. Study on the sensitivities of eight antibiotics on Alexandrium tamarense (Lebour) Balech[J]. Haiyang Xuebao, 2007, 29(2): 123-130.

Citation:

PDF( 755 KB)

Study on the sensitivities of eight antibiotics on Alexandrium tamarense (Lebour) Balech

1.
Laborotary of Marine Genetics and Breeding, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China
2.
National Marine Environmental Monitoring Center, State Oceanic Administration, Dalian 116023, China
3.
Qingdao Growful Pharmaceutical Corpouation Lat., Qingdao 266510, China

Received Date: 2005-08-05
Rev Recd Date: 2006-03-20

Abstract

Abstract

The sensitivities of a toxic strain of Alexandrium tamarense(Lebour) Balech to eig ht antibiotics:chloram phenicol(Cm),erythromy cin(Er),lincomy cin(Lm),gentamicin(Gm),auromycin(Aum),ampicillin(Amp),neomycin(Nm) and strepto mycin(Str) were reported in the present studies.The result revealed that this dinoflag ellate was sensitive to five of the eight antibiotics.The EC50(50% effective concent ration) of specific growth rate after 8 days were 2.45 Lg/cm³,25.2 Lg/cm³,7.95 Lg/cm³,285 u/cm³ and 58.7 Lg/cm³ for Cm,Er,L m,Gm and Aum respectively.The five antibiotics were applicable as selective reagents,and the correspondent genes maybe employed as positive selective markersing enetic engineering of Alexandrium tamarense.On the contrary,Nm and Strhad noinhibition effects on the growth of the algae.These two antibiotics could be used in sterile purification of A.tamarense for clarifying the mechanism of the toxigenicity and the out break of the red tide.The inhibition effect of the selected antiobiotics were further tested.
- Alexandrium tamarense,
- antibiotics,
- genetic engineering,
- selective marker,
- strain sterilization

FullText(HTML)

References(23)

References

齐雨藻,邹景忠,梁松.中国沿海赤潮[M].北京:科学出版社,2003.

周名江,李钧,于仁诚,等.赤潮藻毒素研究进展[J].中国海洋药物,1999,71:48-54.

周名江,颜天,傅萌,等.塔玛亚历山大藻对海产双壳类生命活动的影响[J].海洋学报,2004,26(2):81-86

颜天,谭志军,李钧,等.塔玛亚历山大藻和赤潮异弯藻对黑褐新糠虾和卤虫的急性毒性作用[J].中国海洋大学学报,2004,26(1):76-81.

林元烧.有毒甲藻——塔玛亚历山大藻在厦门地区虾塘引起的赤潮[J].台湾海峡,1996,15(1):16-18.

GU Hai-feng,,LAN Dong-zhao,FANG Qi,et al.Cyst formation,development of Alexandrium tamarense from Yangtse River estuary and its relation to bloom dynamics[J].Acta Botanica Sinica,2004,46(9):1 025-1 031.

KODAMA M,OGATA T,SATO S.Bacterterial production of saxitoxin[J].Agric.Biol Chem,1998,52:1 075-1 077.

YOSHIDA T,KAZUMARU M,TERERUKI C,et al.Purification and characterization of sulfotransferase specific to 0-22 of 11-hydroxy saxitoxin from the toxic dinoflagellate Gymnodinium catenatum (dinophyceae)[J].Fisheries Science,2002,6:634-642.

林伟,周名江.有毒藻产毒过程中海洋细菌的作用[J].海洋科学,2001,25(3):34-38.

ZHANG Z D,GREEN B,CAVALIER-SMITH T.Single gene circles in dinoflagellate chloroplast genomes[J].Nature,1999,400:155-159.

陈其军,肖玉梅,王学臣,等.植物功能基因组研究中的基因敲除技术[J].植物生理学通讯,2004,40(1):121-126.

黄健,唐学玺,宫相忠,等.海洋微藻基因工程的选择标记[J].植物学报,2000,42(8):841-844.

徐轶肖,江天久,吕颂辉.有毒赤潮甲藻塔玛亚历山大藻(香港株Ⅱ)的生长特性研究[J].热带亚热带植物学报,2005,13(1):21-24.

J.萨姆布鲁克,D.W 拉塞尔.分子克隆实验指南(第三版)[M].金冬雁等译.北京:科学出版社,2002.8:1 599.

王镜岩,朱圣庚,徐长法.生物化学[M].北京:高等教育出版社,1991.10.

张亚萍,于文功,戴继勋,等.条斑紫菜叶状体细胞的抗生素敏感性研究[J].青岛海洋大学学报,2002,32(2):245-250.

于道展,杨玲玲,姜鹏,等.裙带菜基因工程选择标记的研究[J].高技术通讯,2003,6:74-77.

耿德贵,王义琴,李文斌,等.杜氏盐藻基因工程选择标记的研究[J].生物技术,2001,11(5):1-3.

臧晓南,张学成,王高歌,等.节旋藻和螺旋藻对7种抗生素敏感性的比较研究[J].海洋学报,2004,26(3):82-92.

PORSCH P.The nonradioactive chloramphenical acetyltransferase enzyme-linked immunosorbent assay test issuited for promoter activity studies in plant protoplasts[J].Anal Biochem,1993,211:113-116.

汪川,樊天,李海辉,等.质粒载体pMG36e在宿主菌中的遗传稳定性研究[J].卫生研究,2005,34(2):214-216.

杨劲松,赵南芸,邹轩,等.肠球菌中抗生素抗性传递的研究[J].海峡预防医学杂志,1998,4(1):12-14.

WANG Cuan-hong,ZHAO Ming-zhen,YU Han-hai,et al.Effect of antibiotic treatment on toxin production by Alexandrium tamarense[J].2003,16(2):340-347.

Relative Articles

Supplements(0)

Cited By

Proportional views