Cloning and analysis of 28S rDNA partial sequences of several strains of Prorocentrum

WANG Bo; MI Tie-zhu; L&#220;Song-hui; SUN Jun; LI Rong-xiu; YU Zhi-gang

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WANG Bo, MI Tie-zhu, LÜSong-hui, SUN Jun, LI Rong-xiu, YU Zhi-gang. Cloning and analysis of 28S rDNA partial sequences of several strains of Prorocentrum[J]. Haiyang Xuebao, 2007, 29(1): 120-126.

Citation:

WANG Bo, MI Tie-zhu, LÜSong-hui, SUN Jun, LI Rong-xiu, YU Zhi-gang. Cloning and analysis of 28S rDNA partial sequences of several strains of Prorocentrum[J]. Haiyang Xuebao, 2007, 29(1): 120-126.

WANG Bo, MI Tie-zhu, LÜSong-hui, SUN Jun, LI Rong-xiu, YU Zhi-gang. Cloning and analysis of 28S rDNA partial sequences of several strains of Prorocentrum[J]. Haiyang Xuebao, 2007, 29(1): 120-126.

Citation:

WANG Bo, MI Tie-zhu, LÜSong-hui, SUN Jun, LI Rong-xiu, YU Zhi-gang. Cloning and analysis of 28S rDNA partial sequences of several strains of Prorocentrum[J]. Haiyang Xuebao, 2007, 29(1): 120-126.

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Cloning and analysis of 28S rDNA partial sequences of several strains of Prorocentrum

1.
Ocean University of China, Qingdao 266003, China
2.
Institute of Hydrobiology, Jinan University, Guangzhou 510632, China
3.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
4.
Shanghai Jiaotong University, Shanghai 200030, China

Received Date: 2005-03-22
Rev Recd Date: 2005-11-03

Abstract

Abstract

The 28S rDNA partial sequences of several strains of Prorocentrum were cloned and sequenced, and other 14 corresponding sequences of Prorocentrum were obtained from Genbank.Then the phylogenetic trees of Prorocentrum were constr ucted with the methods of NJ(neighbour-joining) and M E(minimume-volution).The results demonstrated that the sequenced partial 28S rDNA of 7 strains was 950~958 bp.The phylogenetictree based on the NJ method was similar with that based on the ME method.The sequences of the different strains within the same species are highly conservative;other wise, the diversity among the sequences from different species was obvious.P.micans separated from the South China Sea was very different from the P.micans strains separated from other area of the world, and the dist ances between them were even long erthan the distances between two species.The sequences from the 28S rDNA would be helpful to design species-specific probes of the phytoplankton.
- Prorocentrum,
- 28S rDNA,
- phylogenetic analysis

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References(30)

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