Qualitative and quantitative detection of <i>Prorocentrum minimum</i> with double specific probes assay

CAI Qing-song; LI Rong-xiu; ZHEN Yu; MI Tie-zhu; YU Zhigang

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CAI Qing-song, LI Rong-xiu, ZHEN Yu, MI Tie-zhu, YU Zhigang. Qualitative and quantitative detection of Prorocentrum minimum with double specific probes assay[J]. Haiyang Xuebao, 2006, 28(6): 127-133.

Citation:

CAI Qing-song, LI Rong-xiu, ZHEN Yu, MI Tie-zhu, YU Zhigang. Qualitative and quantitative detection of Prorocentrum minimum with double specific probes assay[J]. Haiyang Xuebao, 2006, 28(6): 127-133.

CAI Qing-song, LI Rong-xiu, ZHEN Yu, MI Tie-zhu, YU Zhigang. Qualitative and quantitative detection of Prorocentrum minimum with double specific probes assay[J]. Haiyang Xuebao, 2006, 28(6): 127-133.

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Qualitative and quantitative detection of Prorocentrum minimum with double specific probes assay

1.
Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;Graduate School, Chinese Academy of Sciences, Beijing 100039, China
2.
School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200033, China
3.
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China

Received Date: 2005-01-10
Rev Recd Date: 2005-11-16

Abstract

Abstract

Two groups of oligonucleotide probes targeted respectively large subunit ribosomal RNA and small subunit ribosomal RNA of Prorocentrum minimum,and the eight species of harmful algae were in cluded to decide the specificity of the probes.In order to get better result,some experiment conditions such as the time of lysating the algae cells with ultrasonic and the time digesting the DNA/RNA hybrids with nuclease S1.The results showed that the probes LSU targeted large subunit ribosomal RNA were specific to Porocentrum minimum,the colorint ensity is positively linear related to the cellular number,and the detection limitis low to 13 cells.Although the probes SSU targeted the small subunit ribosomal RNA were specific to most harmful algae,they seriously cross reacted with Prorocentr umdentatum due to the high homology between the small subunit ribosomal RNA of P.minimum and P.dentatum.Such,the technology is am enable to automation and can be used to monitor the low density of P.minimum in flied sample in long-term program.
- rRNA,
- nuclease protection assay in tegrated with sandwich hybridization,
- Prorocentrum minimum

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