The identification of <i>Epinephelus malabaricus</i> and <i>E.coioides</i> based on DNA markers

DU Jia-ying; DING Shao-xiong; WANG Jun; WANG Shi-feng; ZHUANG Xuan; YOU Ying-zhe

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Article Navigation > Haiyang Xuebao > 2006 > 28(6): 94-99

DU Jia-ying, DING Shao-xiong, WANG Jun, WANG Shi-feng, ZHUANG Xuan, YOU Ying-zhe. The identification of Epinephelus malabaricus and E.coioides based on DNA markers[J]. Haiyang Xuebao, 2006, 28(6): 94-99.

Citation:

DU Jia-ying, DING Shao-xiong, WANG Jun, WANG Shi-feng, ZHUANG Xuan, YOU Ying-zhe. The identification of Epinephelus malabaricus and E.coioides based on DNA markers[J]. Haiyang Xuebao, 2006, 28(6): 94-99.

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The identification of Epinephelus malabaricus and E.coioides based on DNA markers

1.
Department of Oceanography and Institute of Subtropical Oceanography, Xiamen University, Xiamen 361005, China
2.
Zhangzhou Aquaculture Technigue Popularizing Department, Zhangzhou 363000, China

Received Date: 2006-02-26
Rev Recd Date: 2006-05-10

Abstract

Abstract

Using multi-molecular marker technology and based on morphological criteria,the genetic relationship between E.malabaricus and E.coioides was examined in the hope of resolving the long standing issue of identifying of these two species.Results showed that:(1) E.coioides and E.malabaricus should be identified as two species,the consistency of mitochondrial DNA cy to chrome b gene sequence between E.malabaricus and E.coioides is 94.4%,the genetic similarity by AFLP was 0.7539;(2) hybridization exists between E.malabaricus and E.coioides,the specific RAP D and AFLP frag ments are found to be useful in the ident ification of these two species,the genetic properties(both with exterior and inheritance) of hybrid is significantly biased to the male parents;and(3) AFLP was a potentially powerful tool in constructing genetic linkage map for these two groupers.
- E.malabaricus,
- E.coioides,
- hybridize,
- molecular identification

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