The preliminary studies of a lectin(CFL) from <i>Charybdis feriatus</i> Ⅱ.The molecular composition and the characterization of CFL

DAI Cong-jie; WANG Gui-zhong; LI Shaojing; AI Chun-xiang

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DAI Cong-jie, WANG Gui-zhong, LI Shaojing, AI Chun-xiang. The preliminary studies of a lectin(CFL) from Charybdis feriatus Ⅱ.The molecular composition and the characterization of CFL[J]. Haiyang Xuebao, 2006, 28(3): 98-102.

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DAI Cong-jie, WANG Gui-zhong, LI Shaojing, AI Chun-xiang. The preliminary studies of a lectin(CFL) from Charybdis feriatus Ⅱ.The molecular composition and the characterization of CFL[J]. Haiyang Xuebao, 2006, 28(3): 98-102.

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The preliminary studies of a lectin(CFL) from Charybdis feriatus Ⅱ.The molecular composition and the characterization of CFL

1.
Department of Oceanography, Xiamen University, Xiamen 361005, China;Key Lab oratory Marine Environmental Sciences of Ministry of Education, Xiamen University, Xiamen 361005, China;Department of Biology, Quanzhou Normal College, Quanzhou 362000, China
2.
Department of Oceanography, Xiamen University, Xiamen 361005, China;Key Lab oratory Marine Environmental Sciences of Ministry of Education, Xiamen University, Xiamen 361005, China

Received Date: 2005-07-01
Rev Recd Date: 2005-09-14

Abstract

Abstract

Lectin of Charybdis feriatus(shortly call CFL)was studied by biochemistry methods.The results show that the lectin agglutinates every kinds RBC from different animals and microbial cells tested widely in the experiment,as a Ca²⁺ dependent lect in CFL is inhibited by various sugars.CFL is sensitive to the temperature over 40 e and to the pH over 9.0 or below 5.0.No matter adding or not adding-S-S-reducant to the sample of CFL,it demonstrated one single band on plate SDS-PAGE.It shows that CFL does not contain-S-S-,and its molecular mass is 40.8kDa.The analysis result of aminoacid composition of CFL indicates that acidity amino acids were abundant.
- CFL(lectin from crab Charybdis feriatus),
- sugar inhibition,
- thermost ability,
- molecula weight,
- aminoacid composition

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References(20)

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