The cloning and sequence analysis of the 5.8S rDNA and its region from three strains of marine diatoms

ZHANG Bao-yu; WANG Guang-ce; L&#220; Song-hui; QI Yu-zao; ZOU Jing-zhong; ZENG Ceng-kui

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ZHANG Bao-yu, WANG Guang-ce, LÜ Song-hui, QI Yu-zao, ZOU Jing-zhong, ZENG Ceng-kui. The cloning and sequence analysis of the 5.8S rDNA and its region from three strains of marine diatoms[J]. Haiyang Xuebao, 2006, 28(1): 111-117.

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The cloning and sequence analysis of the 5.8S rDNA and its region from three strains of marine diatoms

1.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Graduate School, Chinese Academy of Sciences, Beijing 100039, China
2.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
3.
Institute of Hydrobiology, Jinan University, Guangzhou 510632, China

Received Date: 2004-02-16
Rev Recd Date: 2004-08-19

Abstract

Abstract

Pseudo-nitzschia pungens and Skeletonema costatum are two strains of diatoms which cause harmful algal bloom in China.SK-1 and SK-2 are tw o strains of diatoms,assumably species of S.costatum by morphological characteristics.Three strains,SK-1 which was isolated from the East China Sea and SK-2 which was isolated from the Aquatic Farm of Qing dao and one strain of Pseudo-nitzschia pungens which was from the East China Sea,were st udied based on rDNA data.The sequences of 5.8S rDNA and ITS regions of three strains of diatoms were amplified,cloned and sequenced.Meanwhile,seven additional ITS sequences and 5.8S rDNA were included in the data analysis.The results were as follows:(1) The ITS sequence and 5.8S rDNA of P.pungens were 693 bp while the sequence of SK-1 was 715 bp,which contained complete ITS and 5.8S rDNA,partial 18S rDNA and 28S rDNA;The ITS and 5.8S rDNA sequences of SK-2 were 331bp;(2) the sequence of P.pungens had very high level of similarity with that of two strains of P.pungens retrieved from GenBank,and the similarity was 100%;while it showed 82.9% of identity compared with P.multiseries which also belonged to the genus of Pseudo-nitzschia;the ITS sequence similarity of SK-2 and SK-1 was very low,showing 51.5% of identity,but it had very high level of similarity with that of Skeletonema pseudocostatum(95.5%) ;the ITS sequence of SK-1 had lower similarity with S.pseudocostatum;(3) results of evolutionary tree coincided with similarity analysis of all three strains;it was suggested that recognition of P.pungens by morphological characteristics was supported with r DNA and ITS regions;(4) the findings demonst rated that classifications of SK-2 and SK-1 based on morphological criteria maybe were incongruous.SK-2 should be belonged to S.pseudocostatum and more data are needed to classify which groups SK-1 is belonged to.
- diatom,
- Pseudo-nitzschia pungens,
- ITS region,
- sequence analysis,
- phylogeny

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References(30)

References

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