Gynogenetic diploid induced by suppression of first cleavage of <i>Chlamys farreri</i>

PAN Ying; LI Qi; YU Rui-hai; WANG Ru-cai

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PAN Ying, LI Qi, YU Rui-hai, WANG Ru-cai. Gynogenetic diploid induced by suppression of first cleavage of Chlamys farreri[J]. Haiyang Xuebao, 2005, 27(6): 154-161.

Citation:

PAN Ying, LI Qi, YU Rui-hai, WANG Ru-cai. Gynogenetic diploid induced by suppression of first cleavage of Chlamys farreri[J]. Haiyang Xuebao, 2005, 27(6): 154-161.

PAN Ying, LI Qi, YU Rui-hai, WANG Ru-cai. Gynogenetic diploid induced by suppression of first cleavage of Chlamys farreri[J]. Haiyang Xuebao, 2005, 27(6): 154-161.

Citation:

PAN Ying, LI Qi, YU Rui-hai, WANG Ru-cai. Gynogenetic diploid induced by suppression of first cleavage of Chlamys farreri[J]. Haiyang Xuebao, 2005, 27(6): 154-161.

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Gynogenetic diploid induced by suppression of first cleavage of Chlamys farreri

1.
Fisheries College, Ocean University of China, Qingdao 266003, China;College of Animal Science and Technology, Guangxi University, Nanning 530005, China
2.
Fisheries College, Ocean University of China, Qingdao 266003, China

Received Date: 2004-11-18
Rev Recd Date: 2005-09-21

Abstract

Abstract

Conditions required for the induction of gynogenetic diploid by suppression of first cleavage of Chlamys farreri were examined using treatments of cytochalasin B (0.5 g/cm³;CB) and 6-dimethylamin-opurine (60 g/cm³;6-DMAP).Treatments with CB and 6-DMAP were effective in inhibiting not only meiosis but alsomitosis,and the optimum time for the suppression of first cleavage began at 60~65 min with 20 and 15 min duration time after insemination.This resulted in 12.5% and 27.5% mitotic gynogenetic diploids (mitotic-G2N) respectively.Cytological observations indicated that 6-DMAP disrupted the spindle at mitosis and inhibited chromosome movement,resulting in the formation of one diploid female nucleus,while CB inhibited the cytokinesis of first cleavage but not the segregation of chromosomes.Although attempts to produce mitotic-G2N demonstrated that ploidy levels and the developmental rate of D-shaped lar-vaearelow,the results of this study suggest the possibility of producing mitotic-G2N Chalamys farreri.
- Chlamys farreri,
- gynogenetic diploid,
- 6-DMAP,
- CB,
- cytology

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References(26)

References

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