Analysis of fatty acids and sterols in one marine feed microalga and its potential chemotaxonomic significance

XU Ji-lin; YAN Xiao-jun; ZHU Yi-feng; WANG Xiao-shen; YU Hong

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XU Ji-lin, YAN Xiao-jun, ZHU Yi-feng, WANG Xiao-shen, YU Hong. Analysis of fatty acids and sterols in one marine feed microalga and its potential chemotaxonomic significance[J]. Haiyang Xuebao, 2005, 27(4): 121-126.

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Analysis of fatty acids and sterols in one marine feed microalga and its potential chemotaxonomic significance

Key Laboratory of Marine Biotechnology, Ningbo University, Ningbo 315211, China

Received Date: 2003-05-13
Rev Recd Date: 2004-02-06

Abstract

Abstract

There is an unknown microalga(ZM2020) which has been used widely and proved to be nutritious as feed.In order to evaluate its nutrition value based on fatty acids and sterols,the alga has been cultured in two modes as standing and air bubbling conditions,simultaneo us analysis of fatty acids and sterols has been carried out on agas chromatography mass spectrometer by applying a new step-wise derivetization method.The results indicate that the microalg a is abundant in EPA,C14:0,C16:0,C16:1,as well as some conceivable amount of DH A,C22:5(n-6) and C18 unsaturated fatty acids.Eleven C27 to C30 sterols have been identified with major ones including 24-methyl(ethyl)-cholesterol.The existence of unusual sterols of methylpavlovol and ethyl pavlovol has been observed unam biguously.Based on the composition analysis of fatty acids and sterols,this alga can be classified into Prymnesiophy ceae,Pavlovales,Pavlo vaceae.It is also demonstrated here that lipid analysis can be a useful tool for microalgal chemotaxonomy.
- fatty acids,
- sterols,
- marine feed microalgae,
- chemotaxonomy

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References(17)

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