Culture medium optimization for pigment production with RSM method

WU Hong-qing; TIAN Li; ZHANG Jiu-ming; WEN Zhan-bo

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WU Hong-qing, TIAN Li, ZHANG Jiu-ming, WEN Zhan-bo. Culture medium optimization for pigment production with RSM method[J]. Haiyang Xuebao, 2005, 27(3): 142-146.

Citation:

WU Hong-qing, TIAN Li, ZHANG Jiu-ming, WEN Zhan-bo. Culture medium optimization for pigment production with RSM method[J]. Haiyang Xuebao, 2005, 27(3): 142-146.

WU Hong-qing, TIAN Li, ZHANG Jiu-ming, WEN Zhan-bo. Culture medium optimization for pigment production with RSM method[J]. Haiyang Xuebao, 2005, 27(3): 142-146.

Citation:

WU Hong-qing, TIAN Li, ZHANG Jiu-ming, WEN Zhan-bo. Culture medium optimization for pigment production with RSM method[J]. Haiyang Xuebao, 2005, 27(3): 142-146.

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Culture medium optimization for pigment production with RSM method

1.
Ley Lab oratory of Marine Biological Active Substance, First Institute of Oceanography, Qingdao 266061, China;Hebei Univerity of Technology, Tianjin 300130, China
2.
Ley Lab oratory of Marine Biological Active Substance, First Institute of Oceanography, Qingdao 266061, China;Qindao University of Science and Technology, qindao 266042, Chian
3.
Ley Lab oratory of Marine Biological Active Substance, First Institute of Oceanography, Qingdao 266061, China

Received Date: 2003-03-18
Rev Recd Date: 2003-06-09

Abstract

Abstract

Response surface methodology was used to optimize a medium for pigment production of marine bacterium S-9801 strain.In the first optimization step the influence of yeast extract,peptone,glucose and sodium chloride on pigment production was evaluated using a fractional factorial design.Glucose and sodium chloride influenced pigment production positively while other components had no significant effect.The path of steepest ascent was used to approach the optimal region of the medium composition.In the third step the optional concent ration of glucose and sodium chloride was determined by a central composite design and response surface analysis.The optimized medium allowed pigment production(OD_535~650) to be in creased from 463 to 559,320% higher than the original medium.
- response surface methodology,
- red pigment,
- optimization

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References(9)

References

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