Studies on the larval identification and taxonomy of <i>Lutjanus</i> by AFLP technology

ZHANG Jun-bin; HUANG Liang-min; CHEN Zhen-ran

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ZHANG Jun-bin, HUANG Liang-min, CHEN Zhen-ran. Studies on the larval identification and taxonomy of Lutjanus by AFLP technology[J]. Haiyang Xuebao, 2005, 27(2): 165-171.

Citation:

ZHANG Jun-bin, HUANG Liang-min, CHEN Zhen-ran. Studies on the larval identification and taxonomy of Lutjanus by AFLP technology[J]. Haiyang Xuebao, 2005, 27(2): 165-171.

ZHANG Jun-bin, HUANG Liang-min, CHEN Zhen-ran. Studies on the larval identification and taxonomy of Lutjanus by AFLP technology[J]. Haiyang Xuebao, 2005, 27(2): 165-171.

Citation:

ZHANG Jun-bin, HUANG Liang-min, CHEN Zhen-ran. Studies on the larval identification and taxonomy of Lutjanus by AFLP technology[J]. Haiyang Xuebao, 2005, 27(2): 165-171.

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Studies on the larval identification and taxonomy of Lutjanus by AFLP technology

1.
South China Sea Institute of Oceanography, Chinese Academy of Sciences, Guangzhou 510301, China;Institute of Oceanography, Chinese Academy of Sciences, Qingdao 266071, China
2.
South China Sea Institute of Oceanography, Chinese Academy of Sciences, Guangzhou 510301, China

Received Date: 2003-10-06
Rev Recd Date: 2004-07-26

Abstract

Abstract

The Nansha Coral Reefs(also called Spratly Archipelago)water area,which is located in the South China Sea,is the main habitat and spawn area for the species of Lutjanus in China.The larvae of many closely related species,such as those of the genus Lutjanus,are different to be distinguished mor-phologically.APCR-based fingerprinting technology called amplified fragment length polymorphism (AFLP)was used in the characterization and identification of 11 species of the genus Lutjanus sampled from the Nansha Coral Reefs.The optimal AFLP pattern was obtained with primer combination of E+AGC/M+CAA selective nucleotides.Totally,132 AFLP loci were found from these species and the number of AFLP bands for each species varied from 43 to 69.More than 90% of AFLP bands were species-specific and only 7 markers were fixed to all species examined.All larvae were successfully identified to 11 Lutjanus species by comparing AFLP finger prints of larvae with that of adults.Moreover,the phylogenetic analysis(Neighboring)of species relationship based on the AFLP data was compared with the current morphological taxonomic data.It suggests that AFLP analysis is a reliable method for the larval identification and taxonomy of the genus Lutjanus.
- AFLP,
- larval identification,
- taxonomy,
- genus Lutjanus

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References(21)

References

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