Cryopreservation of clonal gametophytes of <i>Undaria pinnatifida</i> by encapsulation-dehydration

WANG Qi-hua; LIU Yan-ping; ZHANG En-dong; LI Da-peng; ZHANG Lan-cui

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WANG Qi-hua, LIU Yan-ping, ZHANG En-dong, LI Da-peng, ZHANG Lan-cui. Cryopreservation of clonal gametophytes of Undaria pinnatifida by encapsulation-dehydration[J]. Haiyang Xuebao, 2005, 27(2): 154-159.

Citation:

WANG Qi-hua, LIU Yan-ping, ZHANG En-dong, LI Da-peng, ZHANG Lan-cui. Cryopreservation of clonal gametophytes of Undaria pinnatifida by encapsulation-dehydration[J]. Haiyang Xuebao, 2005, 27(2): 154-159.

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Cryopreservation of clonal gametophytes of Undaria pinnatifida by encapsulation-dehydration

1.
School of Life Sciences, Liaoning Normal University, Dalian 116029, China
2.
Institute of Oceanology, Chinese (Academy) of Scieneces, Qingdao 266071, China

Received Date: 2004-08-02
Rev Recd Date: 2004-10-13

Abstract

Abstract

Clonal gametophytes of Undaria pinnatifida were encapsulated in 3% calcium alginate beads. The main factors influencing the viability and the developm entability of clonal gametophy tes,such as sucrose concentration,preculture time,dehydration rate,water content of beads and thawing rate were studied.The results show that the high viability can be obtained when alginate beads are precultured for 9 h in the medium containing 0.3 mol/dm³ sucrose.The optimum average dehydration rate of beads is reduction of 5% water content per hour and the optimum water content of beads is 25%.Fast rate of thawing can significantly improve the viability of clonal gametophytes.Under optimum conditions,the cry opreservation viability of clonal gametophytes can reach 66% at the most.After cry opreservation,the clonal game to phytes can produce the normal sporophytes in the conditions suitable for development.
- clonal Undaria pinnatifida,
- cryo preservation,
- encapsulation dehydration,
- viability

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References(21)

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