Cloning and sequence analysis of allophycocyanin genes from <i>Porphyra haitanensis</i>

ZUO Zheng-hong; DENG Yuan-gao; CHEN Yi-xin; ZHAO Yang; ZHENG Wei-yun

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ZUO Zheng-hong, DENG Yuan-gao, CHEN Yi-xin, ZHAO Yang, ZHENG Wei-yun. Cloning and sequence analysis of allophycocyanin genes from Porphyra haitanensis[J]. Haiyang Xuebao, 2005, 27(2): 148-153.

Citation:

ZUO Zheng-hong, DENG Yuan-gao, CHEN Yi-xin, ZHAO Yang, ZHENG Wei-yun. Cloning and sequence analysis of allophycocyanin genes from Porphyra haitanensis[J]. Haiyang Xuebao, 2005, 27(2): 148-153.

ZUO Zheng-hong, DENG Yuan-gao, CHEN Yi-xin, ZHAO Yang, ZHENG Wei-yun. Cloning and sequence analysis of allophycocyanin genes from Porphyra haitanensis[J]. Haiyang Xuebao, 2005, 27(2): 148-153.

Citation:

ZUO Zheng-hong, DENG Yuan-gao, CHEN Yi-xin, ZHAO Yang, ZHENG Wei-yun. Cloning and sequence analysis of allophycocyanin genes from Porphyra haitanensis[J]. Haiyang Xuebao, 2005, 27(2): 148-153.

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Cloning and sequence analysis of allophycocyanin genes from Porphyra haitanensis

1.
Key Laboratory of Ministry of Education on Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China;Environmental Science Research Center, Xiamen University, Xiamen 361005, China
2.
Key Laboratory of Ministry of Education on Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China
3.
Environmental Science Research Center, Xiamen University, Xiamen 361005, China

Received Date: 2003-08-26
Rev Recd Date: 2004-03-08

Abstract

Abstract

On the basis of the sequences of allophycocyanins from several species in genbank,a pair of degenerate primers were designed and synthesized.Aspecific fragment about 1 055 bp in size was obtained after PCR amplification using the cpDNA of Porphyra haitanensis as template.Then the purified fragment of DNA was cloned into vector pMD18-T,and sequenced after the identification of the recombinants using PCR and endonuclease digest.The results indicate that A and B subunit genes of allophy cocyanin are obtained from Porphyra haitanensis.The fragment size is 1 055 bp,including A and B subunits coding regions 486 bp each and the spacer between them is 83 bp.The genes were submitted to genbank and their genbank accession number was AY372218.
- Porphyra haitanensis,
- allophycocyanin,
- gene cloning,
- sequence analyzing

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References(11)

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