The genetic relationship between <i>Neobenedenia girellae</i> and <i>N. melleni</i> inferred from 28S rRNA sequences

ZHANG Wen; WANG Jun; SU Yong-quan; DING Shao-xiong; YANG Wen-chuan

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Article Navigation > Haiyang Xuebao > 2004 > 26(5): 116-122

ZHANG Wen, WANG Jun, SU Yong-quan, DING Shao-xiong, YANG Wen-chuan. The genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences[J]. Haiyang Xuebao, 2004, 26(5): 116-122.

Citation:

ZHANG Wen, WANG Jun, SU Yong-quan, DING Shao-xiong, YANG Wen-chuan. The genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences[J]. Haiyang Xuebao, 2004, 26(5): 116-122.

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The genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences

1.
Department of Oceanography, Xiamen University, Xiamen 361005, China;Institute of Subtropical Oceanography, Xiamen University, Xiamen 361005, China
2.
Deptartment of Biology, Xiamen University, Xiamen 361005, China

Received Date: 2003-02-13
Rev Recd Date: 2003-09-22

Abstract

Abstract

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda,Neobenedenia girellae and N.melleni were obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced.The results show that the comparison of 28S rRNA sequences,with only a base varies in 337 bp accounting for 0.3% genetic difference,the relative species N.girellae and N.melleni parasitized on the different fishes in different farms display that they possess very high genetic similarity of 99.7%,higher than that of 99.41% for the single species N.melleni cultured in different area,and the intraspecific divergence of N.melleni is 0.59%.Meanwhile,the interspecific differences between the two Neobenedenia and three Benedenia (i.e.B.lutjani,B.rohdei and B.seriolae) range from 2.08% to 11.73%.In addition,UPGMA and MP molecular phylogenetic trees were constructed and proved to be consistent with each other.Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show the high similarity,whether they belong to a single species or not is still undefined,the more genes of them should be further investigated,combining with the systematicall and detailed morphological study.
- 28S rRNA,
- Neobenedenia,
- sequence analysis

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References(13)

References

YAMAGUTI S.Systema Helminthum:Vol.Ⅳ.Monogenea and Aspidocotylea [M].New York:John Wiley and Dons Interscience Publishers,1963.699.

WHITTINGTON I D,HORTON M A.A revision of Neobenedenia Yamaguti,1963 (Monogenea Capsalidae) including a redescription of N-melleni (MacCallum,1927) Yamaguti[J].Journal of Natural History,1996,30(8):1 113-1 156.

BONDADREANTASO M G,OGAWA K,FUKUDOME M.Reproduction and growth of Neobenedenia girellae (Monogenea,Capsalidae):a skin parasite of cultured marine fishes of Japan[J].Fish Pathology,1995,30(3):227-231.

KOESHARYANI I,ZAFRAN,YUASA K,et al.Two species of Capsalid monogeneans infecting cultured humpback grouper Cromileptes altivelis in Indonesia[J].Fish Pathology,1999,34(3):165-166.

YOSHINAGA T,NAGAKURA T,OGAWA K,et al.Attachment-inducing capacities of fish tissue extracts on oncomiracidia of Neobenedenia girellae (Monogenea,Capsalidae) [J].Journal of Parasitology,2000,86(2):214-219.

萨姆布鲁克J,弗里奇E F,曼尼阿蒂斯T.分子克隆实验指南(第二版)[M].北京:科学出版社,1995.

HASSOUNA N,MICHOT B,BACHELLERIE J-P.The complete nucleotide sequence of mouse 28S rRNA gene:implications for the process of size increase of the large subunit rRNA in higher eukaryotes [J].Nucleic Acids Res,1984,12:3 563-3 583.

ISABELLE Mollaret,BARRIE G M,JAMIESON Jean-Lou Justine.Phylogeny of the Monopisthocotylea and Polyopisthocotylea (Platyhelminthes) inferred from 28S rDNA sequences[J].International Journal for Parasitology,2001,30:171-185.

PHILIPS R B,PLEYTE K A,BROWN M R.Salmonid phylogeny inferred from ribosomal DNA restriction maps[J].C J Fish Aquat Sxi,1992,49:2 345-2 353.

GERBIS.The evolution of eukaryotic ribosomal DNA[J].Biosystems,1986,19:247-258.

HASEGAWA M,IIDA Y,YANO T,et al.Phylogenetic relationshipis among eucaryotic kingdoms inferred from ribosomal RNA sequences[J].J Mol Evol,1985,22:32-38.

屈良鹄,陈月琴.物分子分类检索表——原理与方法[J].中山大学学报(自然科学版),1999,38(1):1-6.

张纹,王军,苏永全,等.海水养殖鱼类新本尼登虫的RAPD分析[J].海洋学报,2002,24(4):136-140.

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