Comparative study on sensitivities of <i>Arthrospira platensis</i> and <i>Spirulina subsulsa</i> to seven antibiotics

ZANG Xiao-nan; ZHANG Xue-cheng; WANG Gao-ge; LIU Jin-jie; ZHANG Xiao-hui; LIU Bin

Article Contents

Article Navigation > Haiyang Xuebao > 2004 > 26(3): 82-92

ZANG Xiao-nan, ZHANG Xue-cheng, WANG Gao-ge, LIU Jin-jie, ZHANG Xiao-hui, LIU Bin. Comparative study on sensitivities of Arthrospira platensis and Spirulina subsulsa to seven antibiotics[J]. Haiyang Xuebao, 2004, 26(3): 82-92.

Citation:

ZANG Xiao-nan, ZHANG Xue-cheng, WANG Gao-ge, LIU Jin-jie, ZHANG Xiao-hui, LIU Bin. Comparative study on sensitivities of Arthrospira platensis and Spirulina subsulsa to seven antibiotics[J]. Haiyang Xuebao, 2004, 26(3): 82-92.

Citation:

PDF( 1871 KB)

Comparative study on sensitivities of Arthrospira platensis and Spirulina subsulsa to seven antibiotics

Received Date: 2002-07-29
Rev Recd Date: 2003-07-31

Abstract

Abstract

The sensitivities of Arthrospira platensis FACHB341 and Spirulina subsulsa FACHB351 to seven antibiotics-chloramphenicol(Cm),erthromycin(Em),ampicillin(Amp),streptomycin(Str),kanamycin(Km),gentamycin(Gm) and neomycin(Nm) are presented.The results reval that sensitivities of the two species to the seven antibiotics show a similar tendency,however significant difference still exists.It is provedthat the two specis of blue-green algae are all highly sensitive to Em,Cm and Str.0.1 μg/cm³,0.5 μg/cm³ Cm or 5 μg/cm³ is the lethal concentration to them.A.341 and S.351 can be inhibited by 1 μg/cm³ Amp,and then they recovered after 6 d.A. 341 and S.351 are not sensitive to Km,Gm and Nm,Besides the difference of sensitivities between the two algae is great.A.351 cannote inhibited by even 300 μg/cm³ Km,whereas to S.351,50 μg/cm³ Km can obviously in-hibit the growth of it and 200 μg/cm³ Km can kill it.200 μg/ml Gm and 300 μg/cm³ Nm cannotinhibit the growth of these two species.It is suggested that Cm,Em and Strare useful selective markers for genetransfer of A.341 and S.351.It is also shown that the significant difference between A.341 and S.351 maybe is based on their genetic discrepancy.
- Arthrospira platensis,
- Spirulina subsulsa,
- selective marker,
- antibiotics,
- sensitivity

FullText(HTML)

References(23)

References

曹淑莉,向葆卿.8种海洋饵料微藻蛋白质含量及氨基酸组成比例的比较研究[J].海洋学报,1993,15(4):98-103.

陈峰,姜悦.微藻生物技术[M].北京:中国轻工业出版社,1999.

VONSHAK A. Spirulina platensis(Arthrospira):physiology,cell biology and biotechnology[M].Taylor&Francis Ltd,1997.

NELISSEN B,WILMOTTE A,NEEFS J-M,et al. Phylogenetic relationship among filamentous helical cyanobacteria investigated on the basis of 16S ribosomal RNA gene sequence analysis system[J].Appl Microbiol,1994,17:206-210.

茅云翔,杨官品,张宝红,等.16SrRNA基因与16S-23SrRNA转录单元内间隔区序列分析及其在节旋藻和螺旋藻分类鉴定中的应用[J].高技术通讯,2001,6:12-18.

王关林,方宏筠.植物基因工程原理与技术[M].北京:科学出版社,1998.167-174.

THIEL T,POOH. Transformation of a filamentous cyanobacteria by electroporation[J].J Bacterial,1989,171:5 743-5746.

CHAUVAT F,ASTIER C,VEDEL F,et al. Transfornation in the cyanobacterium Synechococcus R2:improvement of efficiency;role of the pUH24 plasmid[J].Mol Gen Genet,1983,191:39-45.

KAREN S Kolowsky,JOHN G K Williams,ALADAR A Szalay. Length of foreign DNA in chimeric plasmids determines the efficiency of its integration into the chromosome of the cyanobacterium Syrnechococcus R2 [J].Gene,1984,27:289-299.

BUZBY J S,PROTER R D,STEVENS S E Jr. Expression of the Escherichia coli LacZ gene on a plasmid vector in a cyanobacterium[J].Science,1985,230:805-807.

MARSAC N T,TORRE F,SZULMAJSTER J. Expression of the larvicidal gene of Bacillus sphaericus 1593M in the cyanobacterium Anacystis nidulans R2[J].Mol Gen Genet,1987,209:396-398.

STEVENS S E,PORTER R D. Transformation in Agmemellum quadruplicatum [J].Proc Natl Acad Sci USA,1980,77:6 052-6 056.

TAKEYAMA H,TAKEKEMURA N,MATSUNAGE T. Transformation of marine cyanobacteria Synechococcus sp. by electroporation. Current Topics in Marine Biotechnology[M].Fuji Technology Press Ltd,198. 159-160.

CAO Ji-xiang,XU Zeng-fu,QIU Guo-hua,et al. Studies on the sensitivity of Spirulina platensis to antibiotics and herbicide:relationship with selectable markers for genetic transformation[J].Bioresource Technology,1999,70:89-93.

王高歌,臧晓南,张宝红,等.螺旋藻对6种抗生素的敏感性研究[J].高技术通讯,2001,7:6-13.

ZARROUK C. Contribution ãl'étude d'une cyanophycée influencéde divers facteurs physiques la croissance et la photosynthése de Spirulina maxima (Setch et Gardner) Geitler[D].Paris:University of Paris,1966.

萨姆布鲁克(J),弗里奇E F,曼尼阿蒂斯T.分子克隆实验指南(第二版)[M].北京:科学出版社,1996.912.

LU F,VONSHAIC A,BOUSSIBA S,et al. Effect of temperature and irradiance on growth of Haematococcus pluvia(Chlorophyceae) [J].J Phycol,1994,30:829-833.

NAP J P,BIJVOET J,STIEKEMA W J. Biosafety of kanamycin-resistant transgenic plants[J].Transgenic Res,1992,11:239-249.

METZ J G,NIXON P J,ROGNER M,et al. Directed alteration of the D1 polypeptide of photosystem. Ⅱ. evidence that tyrosine-161 is the redox component,Z. connecting the oxygen-evolving complex to the primary electron donor P680[J].Biochemistry,1989,28:6 960-6 969.

MURPHY R C,GASPARICH G E,BRYANT D A,et al. Nucleotide sequence and further characterization of the Synechococcus sp. Strain PCC7002 recA gene:complementation of a cyanobacterial recA mutation by Escherichia coli recA gene[J].J Bact,1990,172:967-976.

Van der PLAS J,HEGEMAN H,de VRIEZE G,et al. Genomic intergration system based on pBR322 sequences for the cyanobacterium Synechococcus sp. PCC7942:transfer of genes encoding plastocyanin and ferredoxin[J].Gene,1990,95:39-48.

WU X,VENNISON S J,LIU H,et al. Mosquito larvicidal activity of transgenic Anabaena strain PCC7120 expressing combinations of genes from bacillus thuringiensis subspisraelensis[J].Appl Envir Microbiol,1997,63:4 971-4 975.

Relative Articles

Supplements(0)

Cited By

Proportional views