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The antagonistic protein production, which inhibits 6 species fungi and bacteria from marine Bacillus spp., and the cultural conditions such as temperature, fermentation peried, NaCl concentration and pH of 4 strians marine bacter ia are studied.The result of test shows that suittable factor for both bacteria growth and antimicrobial substance produce are 20~28, 84~108 h, NaCl 1%~3%, pH5~8.Concentration of ammonium sulfate between 50% and 70% is the most fitting saturation for antagonistic protein precipitability.The proteins are insensitive to heat and protein and have high inhibitory value to pathogenetic fungi.