Construction of computerized DNA fingerprinting for identification of <i>Porphyra</i> lines

JIA Jian-hang; CHEN Yi-hua; SHI Jin-feng; JIN De-min; XU Pu; MEI Jun-cue; WENG Man-li; WANG Bin

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Article Navigation > Haiyang Xuebao > 2001 > 23(1): 79-84

JIA Jian-hang, CHEN Yi-hua, SHI Jin-feng, JIN De-min, XU Pu, MEI Jun-cue, WENG Man-li, WANG Bin. Construction of computerized DNA fingerprinting for identification of Porphyra lines[J]. Haiyang Xuebao, 2001, 23(1): 79-84.

Citation:

JIA Jian-hang, CHEN Yi-hua, SHI Jin-feng, JIN De-min, XU Pu, MEI Jun-cue, WENG Man-li, WANG Bin. Construction of computerized DNA fingerprinting for identification of Porphyra lines[J]. Haiyang Xuebao, 2001, 23(1): 79-84.

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Construction of computerized DNA fingerprinting for identification of Porphyra lines

1.
Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China
2.
Jiangsu Institute of Oceanology and Hydrobiology, Nantong 226007, China
3.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

Received Date: 2000-06-02
Rev Recd Date: 2000-08-14

Abstract

Abstract

RAPD(randomly amplified polymorphic DNA) analysis was performed with filaments of 15 Porphyra lines representing P.Yezoensis,P.Haitanensis,P.Katadai var.hemiphylla and P.oligospermatangia.Eight stable and repeatable RAPD bands amplified with two primers,OPN-02 and OPJ-18,were selected for the construction of DNA fingerprinting.The RAPD results are scored based on the presence or absence of each of the 8 bands and then converted to wmputer language expressed with two digitals,1 and 0,which represent the presence(numbered as 1) or absence(numbered as 0) of each band,respectively.Based on these results,a model DNA fingerprint and a cqmputerized DNA fingerprint are constructed.In the constructed DNA fingerprint,each Porphyra line has its unique fingerprinting pattern and can be easily distinguished from each other.Later,a software,named as PhGI,is designed based on this DNA finaerorintina for Porphyra line identification.
- DNA fingerprinting,
- germplasm identification,
- PAPD,
- Porphyra

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References(10)

References

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