Genetic variation analysis of two mullet populations through randomly amplified polymorphic DNA(RAPD) method

RAPD method is explored to investigate the genetic variation between cultivated population(CP) and natural population(NP) of mullet (Liza haematocheila) distributed in waters of the Huanghe River Estuary.Using 11 decamer random primers,112 RAPD sites are detected in the 39 fishes.The high percentage of polymorphic sites 83.93% in CP,85.71% in NP) and the high genetic diversity index (H) (0.212 4 in CP,0.2271 in NP) show rich genetic diversity in the two mullet populations.The big genetic similarity index (F=92.82%) and the small genetic distance (D=0.0718) indicate that there is not obvious genetis differentiation between the two populations,which suggests that aquaculture has generated little reduction in genetic diversity of mullet in this area.The results suggest that aquaculture of mulleta will be a promising industry in the area of the Huanghe River Estuary.