Molecular identification of red tide toxic <i>Alexandrium tamarense Aexandrium catenella</i> from the South China Sea

Chen Yueqin; on Lianghu; Zeng Longmei; Qi Yuzao; Zheng Lei

Article Contents

Article Navigation > Haiyang Xuebao > 1999 > 21(3): 106-112

Chen Yueqin, on Lianghu, Zeng Longmei, Qi Yuzao, Zheng Lei. Molecular identification of red tide toxic Alexandrium tamarense Aexandrium catenella from the South China Sea[J]. Haiyang Xuebao, 1999, 21(3): 106-112.

Citation:

Chen Yueqin, on Lianghu, Zeng Longmei, Qi Yuzao, Zheng Lei. Molecular identification of red tide toxic Alexandrium tamarense Aexandrium catenella from the South China Sea[J]. Haiyang Xuebao, 1999, 21(3): 106-112.

Citation:

PDF( 449 KB)

Molecular identification of red tide toxic Alexandrium tamarense Aexandrium catenella from the South China Sea

1.
School of Life Sciences, Zhongshan University, Guangzhou 510275
2.
School of Chemistry and Chemical Technology, Zhongshan University, Guangzhou 510275
3.
Institute of Hydrobiology, Jinan University, Guangzhou 510632

Received Date: 1997-11-07
Rev Recd Date: 1998-11-05

Abstract

Abstract

The rDNA internal transcribed spacer and 5.8s regions of 7 strains of Alexandrium "tamarense complex" were amplified with the method of PCR amplification with individual cells for RFLP analysis and sequensing. PCR-RFLP analysis of rDNA ITS using three restriction enzymes revealed 7 strains carried unique restriction patterns and were in common with those of Japanese A.catenella.The sequence comparisons of three strains(ADOl, AHOl, ANO1) with the homologous sequences of Japanese A. catenella and A.tamarense determined by Masao Adachi et al.in 1996 showed that three strains from the South China Sea with those Japanese A.catenella were closely related to each other, distance values between strains were extremely low(<0.01), whereas distance values between three strains and Japanese A.tamarense were high(about 0.20), which suggested that 7 strains from the South China Sea were the species of A.catenella.It was concluded that rDNA ITS region could served as criteria for the "red tide" species identification.
- Molecular identification,
- Aleaandrium tamarense

FullText(HTML)

References(16)

References

Balech E.The genus Alezandrium or Gonyaulax of the tamarensis group.In:Toxic Dinoflagellates(Anderson D M,White A W,Baden D G eds),Elsevier,New York,1985,33-38

Taylor F J R.The taxonomy and relationships of red tide dinoflagellates.In:Toxic Dinoflagellates.(Anderson D M,White A W,Baden D G eds),Elsevier,New York,1985,11-26

Adachi M,Sako Y,Ishida Y.The identification of conspecific dinoflagellates Alezandrium tamareuse from Japan and Thailand by monoclonal antibodies.Nippon Suisan Gakkaishi, 1993,59,327-332

Sako Y,Adachi M,Ishida Y.Preparation and characterization of monoclonal antibodies to Alezaudrium species.In:Toxic Phytoplankton Blooms in the Sea(Smayda T J,Shimizu Y eds),Elsevier,Amsterdam,1993,87-93

Cembella A D, Taylor F J R.Electrophoretic variability within the Protogou}aaulaz tamareuse/catenella species complex; pyridine linked dehydrogenases.Biochem.Syst.Ecol.,1989,15,171-186

Hayhome B A,Anderson D M,Kulis D M et al.Variation among congeneric dinoflagellates from the north earstern United States and Canada.Mat.Biol.(Betl.),1985,101,427-435

Qu L H,Hardman N,Gill L L et al.Phylogeny of helminths determined by rRNA sequence comparison.Mol.Biochem Parasitol,1986,20,93-99

Lenaers G,Scholin G A,Bhaud Y et al.A molecular phylogeny of dinoflagellate protists(Pyrrhophyta) inferred from the sequence of 24S rDNA divergent domains Dl and D8.J.Mol.Evol.,1991,32,53-63

Gonzalez I L,Sylvester J E,Smith T F et al.Ribosomal RNA gene sequences and hominoid phylogeny.Mol.Biol.Evol.,1990,7,203-219

Baldwin B G.Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants:an example from the composite.Mol.Phylogen.Evol.,1992,(1):3-16

Morales V M,Pelcher L E,Taylor J L.Comparison of the 5.85 rDNA and internal transcribed spacer sequences of isolates of Leptosphaeria maculans from different pathogenicity groups.Curr.Genet.,1993,23,490-495

Bakker F T,Olsen J L,Stam W T et al.Biogeogtaphy of Cladophoropsis membranacea(Chlorophyta) based on comparisons of nuclear rDNA ITS sequences.J.Phycol.,1992,28,660-668

Adachi M,Sako Y, Ishida Y.Restriction fragment length polymorphism of ribosomal DNA internal transcribed spacer and 5.8s regions in Japanese Alezandrium species(Dinophyceae).J.Phycol.,1994,30,857-63

Adachi M,Sako Y, Ishida Y.Analysis of Alezandrium(Dinophyceae) species using sequences of the S.Ss ribosomal DNA and internal transcribed spacer regions.J.Phycol.,1996,32,424-432

陈月琴,屈良鸽,邱小忠等.甲藻单个细胞DNA的制备及在赤潮藻类分子鉴定中的应用.中山大学学报(自然科学版),1997,36(4):66-69

萨姆布鲁克J.,弗里奇E.F.,曼尼阿蒂斯T.著,金冬雁等译.分子克隆实验指南.第二版.北京:科学出版社.1996

Relative Articles

Supplements(0)

Cited By

Proportional views