Studies on phycobiliprotein in Gracilaria Ⅱ.Characterization on aborption and emission spera and fluorescent dynamics in intact thallus of Gracilaria lemaneiformis

In the present paper, intact living thalli of Gracilaria lemaneiformis,used to measure room temperature.absorption spectra, low temperature (77 K) fluorescent emission spectra, and chlorophyll-inducing fluorescent dyosmic curve.For wild type material (Wt) and yellow mutant (Ye100),there are 7 peaks in the absorption spectra:435. 498. 540. 56B. 618. 640 and 680 nm, and for green mutants Gr180 and Gr184, there are aV the absorption peaks except 540 nm one. The phymerythrin content in three mutants is lower than that in wild type thallus, the comparative content of phycocyanin in gree.mutants is higher than that in others, but there is no obvious change of allophycocyanin content in all the materials. The results of bw temperature (77 K) fluorescent emission indicate that there is no difference for fluorescent emission peaks of the wild type and mutants (the excitation wavelength are 436, 540 and 580 nm respectively,but the transmission effciency of light energy of phycoerythrin in wild type is lower than that of others. The chlorophyll-inducing fluorescent dynamic curve shows that the activity oI PSⅡ,the trans-mission efficiency in primary light energy and the carbon metabolic activity of PSⅡ in wild type thallus are higher than these in three mutants.