Isolation and purification of somatolactin and its N-terminal amino acid sequence analysis from Japanese sea bass (<i>Lateolabrax japonicus</i>)

Xu Bin; Zhang Peijun; Miao Hongzhi; Li Deshang; Shunsuke Moriyama; Hiroshi Kawauchi

Article Contents

Article Navigation > Haiyang Xuebao > 1998 > 20(2): 83-89

Xu Bin, Zhang Peijun, Miao Hongzhi, Li Deshang, Shunsuke Moriyama, Hiroshi Kawauchi. Isolation and purification of somatolactin and its N-terminal amino acid sequence analysis from Japanese sea bass (Lateolabrax japonicus)[J]. Haiyang Xuebao, 1998, 20(2): 83-89.

Citation:

Xu Bin, Zhang Peijun, Miao Hongzhi, Li Deshang, Shunsuke Moriyama, Hiroshi Kawauchi. Isolation and purification of somatolactin and its N-terminal amino acid sequence analysis from Japanese sea bass (Lateolabrax japonicus)[J]. Haiyang Xuebao, 1998, 20(2): 83-89.

Citation:

Xu Bin, Zhang Peijun, Miao Hongzhi, Li Deshang, Shunsuke Moriyama, Hiroshi Kawauchi. Isolation and purification of somatolactin and its N-terminal amino acid sequence analysis from Japanese sea bass (Lateolabrax japonicus)[J]. Haiyang Xuebao, 1998, 20(2): 83-89.

PDF( 2648 KB)

Isolation and purification of somatolactin and its N-terminal amino acid sequence analysis from Japanese sea bass (Lateolabrax japonicus)

1.
Open Laboratory on Aquacultural Research of the State Educational Committee of China, Ocean University of Qingdao, Qingdao 266003
2.
Experimental Marine Biulogioal Luaboratory, Institute of Oceanology, Acudemy of Sciences of Chints.Qingdao 266071
3.
Marine Life College, Oean University of Qingdao, Qingdao 266003
4.
School of Fisheries Scienceic, Kitasuto Clniversity, Sanriku, Iwate 02201, Japan

Received Date: 1997-03-10
Rev Recd Date: 1997-06-13

Abstract

Abstract

Somatolactin(SL),a newly discovered protein pituitary hormone belonging to the GH/PRL family,was first isolated from Japanese sea bass (Lateolabrar japonicus) pituitary in this study.The somatolactin of Japanese sea bass (sbSL) was extracted under alkaline condition from its pituitary glands,and subsequently purified by gel filtration on a Sephadex G-100 column and reversed-phase high performance chromatography (rpHPLC).Japanese sea bass somatolactin (sbSL) was confirmed by the specific reaction with the antibody against chum salmon somatolactin (sSL) through western blotting.The sbSL was found to consist of two forms with molecular weights of 23.2×10³ and 28.4×10³u in SDS-polyacrylamide gel electrophoresis (SDSPAVE),the later may contain a form of glycosylation.Sixteen N-terminal amino acid sequence of sbSL was determined by the dansyl method according to Edman.The sixteen amino acid sequences of sbSL show a higher homology compared to those of known teleost somatolactins,which further revealed that the isolated and purified pituitary hormone was Japanese sea bass somatolactin.
- Japanese sea bass,
- Lateolabrar japonicus,
- somatolactin (SL),
- isolation,
- amino acid sequencing

FullText(HTML)

References(16)

References

Ono M et al.cDNA cloning of somatolactin,a pituitary protein to growth hormone and prolactin.Proc.Natl.Acad.Sci.USA,1990.87,4 330-4 334

Rand-Weaver M et al.Isolation and characterization of somatolactin,a new protein related to growth hormone and prolactin from Atlantic C;od (Gadus morhua) pituitary glands.Biochemistry,1991a,30.1 509-1 515

Rand-Weaver M et al.Somatolactin.a novel pituitary protein:purification and plasma levels during reproductive maturation of coho salmon.J.Endocrinol.,1992,133,393-403

Zhu Y.Thomas P.Elevation of somatolactin in red drum plasma following transfer to a dark background tank.Am.Zool.,1994,34 (42A):

Cavari B F et al.Somatolactin.a novel pituitary protein:Isolation and characterization from Sparus aurata.Mol.Mar.Riol.Biotechnol.1994,4(2):117122

Takayama Y et al.Great conservation of somatolactin.a presumed pipuitary hormone of the growth hormone/prolactin family.than of growth hormone in teleost fish.Gen.Comp.Endocrinol.,1991,83,366-374

Iraqi F et al.Isolation and characterization of somatolactin genes from two cold water teleost,lumpfish(Gyclopterus lumpus) and halibut (Hippoglossus hippoglossus).Mol.Mar.Biol.Biotechnol.,1993,2(2):96-103

Pendon C et al.Cloning of a somatolxctin-encoding cDNA from sole (Soles senegalensis).Gene.1994.147,227-230

Sogahe Y et al.Molecular cloning of somatolactin in the sturgeon,a Chondrostean.3rd internatl.Symp.on Fish Endocrinol.Abstract.,1996.115

Chenq K W et al.Iloninq and characterization of complementary DNA encoding somatolactin in goldfish,Carassius auratus.;ird internatl.Symp.on Fish Endocrinol.Ahstract.1996,116

Rand Weaver M et al.Cellular localization of somatolacin in the pas intermedia of some teleost fishes.Cell Tissue Res.,1991b.263.207-215

Laemmh U K.Cleavage of structural proteins during the assembly of the head of bacterio-phase T4.Nature (London),1970.227,680-685

Rand Weaver M rt al.The complete amino acid sequences of two variants of growth hormone from Atlantic cod (Gadus morhua).Gen.Comp.variants of growth hormone from Atlantic cod (Gadus morhua).C:en.Comp.Endocrinol.1991c,73.260-269

Weber K et al.The reliability of molecular meiqht determined by dodecyl sulfate-polyacrylamide gel electrophoresis.J.Biol.C'hem.、1969,244.406-4 412

Towbin H et al.Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets:procedure and some application.Proc.Natl.Acad.Sci.LISA 9,1979,9 350-4354

NoseT et al.Isolation and characterisation of glycosylated and nonglycosylated prolactins from alligator and crocodile.Int.J.Peptide IZes.,1992,39.250-257

Relative Articles

Supplements(0)

Cited By

Proportional views