Distribution of betaine lipids in 14 species of microalgae

Li Yanrong; Mu Tong; Huang Lili; Xu Jilin; Zhou Chengxu; Yan Xiaojun

doi:10.3969/j.issn.0253-4193.2020.12.008

Volume 42 Issue 12

Jan. 2021

Turn off MathJax

Article Contents

Article Navigation > Haiyang Xuebao > 2020 > 42(12): 72-81

Li Yanrong，Mu Tong，Huang Lili, et al. Distribution of betaine lipids in 14 species of microalgae[J]. Haiyang Xuebao，2020, 42(12)：72–81 doi: 10.3969/j.issn.0253-4193.2020.12.008

Citation:

Li Yanrong，Mu Tong，Huang Lili, et al. Distribution of betaine lipids in 14 species of microalgae[J]. Haiyang Xuebao，2020, 42(12)：72–81 doi: 10.3969/j.issn.0253-4193.2020.12.008

Citation:

Li Yanrong，Mu Tong，Huang Lili, et al. Distribution of betaine lipids in 14 species of microalgae[J]. Haiyang Xuebao，2020, 42(12)：72–81 doi: 10.3969/j.issn.0253-4193.2020.12.008

PDF( 894 KB)

Distribution of betaine lipids in 14 species of microalgae

doi: 10.3969/j.issn.0253-4193.2020.12.008

Li Yanrong^{1, 2
,},
Mu Tong¹,
Huang Lili¹,
Xu Jilin^{2
,
,},
Zhou Chengxu²,
Yan Xiaojun²

1.
Ningbo Institute of Oceanography, Ningbo 315832, China
2.
Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315832, China

Received Date: 2019-09-23
Rev Recd Date: 2020-01-08

Available Online: 2021-01-06

Publish Date: 2020-12-25

Abstract

Abstract

The qualitative and quantitative analyses on betaine lipids of microalgae were conducted by means of the quadrupole flight time ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-Q-TOF-MS) using electron spraying ionization. As a result, 133 betaine lipids including 53 DGCC, 41 DGTS and 39 DGTA were identified from 14 species of microalgae in four major phyla. DGCC was the dominant betaine lipid in dinoflagellates and haptophytes, while DGTS was the main betaine lipid in chlorophytes. There were two main types of betaine lipid in diatoms: DGCC in centric diatom and DGTA in pennate diatom. The differences of fatty acid of betaine lipid in marine microalgae were only observed at the phyla and class levels, but at lower taxonomic levels the differences were less stable. DGCC existed in diatoms, dinoflagellates and chlorophytes, which contained C14−C18 saturated and unsaturated fatty acids and C20 and C22 polyunsaturated fatty acids. The difference in DGCC is that saturated C14−C18 fatty acids existed in dinoflagellates, while the fatty acids of odd carbon numbers C19 occurred only in diatoms. The results of description and molecular characterization of betaine lipid in microalgae can be served in future investigation in chemotaxonomy, physiology, ecological role of microalgae and functional properties of these phosphorous-lacking polar lipids.
- microalgae,
- betaine lipids,
- UPLC-Q-TOF-MS

FullText(HTML)

References(27)

References

[1]	Dembitsky V M. Betaine ether-linked glycerolipids: chemistry and biology[J]. Progress in Lipid Research, 1996, 35(1): 1−51. doi: 10.1016/0163-7827(95)00009-7
[2]	Kumari P, Bijo A J, Mantri V A, et al. Fatty acid profiling of tropical marine macroalgae: an analysis from chemotaxonomic and nutritional perspectives[J]. Phytochemistry, 2013, 86: 44−56. doi: 10.1016/j.phytochem.2012.10.015
[3]	Armada I, Hachero-Cruzado I, Mazuelos N, et al. Differences in betaine lipids and fatty acids between Pseudoisochrysis paradoxa VLP and Diacronema vlkianum VLP isolates (Haptophyta)[J]. Phytochemistry, 2013, 95: 224−233. doi: 10.1016/j.phytochem.2013.07.024
[4]	Banskota A H, Stefanova R, Sperker S, et al. New diacylglyceryltrimethylhomoserines from the marine microalga Nannochloropsis granulata and their nitric oxide inhibitory activity[J]. Journal of Applied Phycology, 2013, 25(5): 1513−1521. doi: 10.1007/s10811-012-9967-1
[5]	Laloknam S, Tanaka K, Buaboocha T, et al. Halotolerant cyanobacterium Aphanothece halophytica contains a Betaine transporter active at alkaline pH and high salinity[J]. Applied and Environmental Microbiology, 2006, 72(9): 6018−6026. doi: 10.1128/AEM.00733-06
[6]	Nakanishi K, Deuchi K. Culture of a high-chlorophyll-producing and halotolerant Chlorella vulgaris[J]. Journal of Bioscience and Bioengineering, 2014, 117(5): 617−619. doi: 10.1016/j.jbiosc.2013.10.024
[7]	Bigogno C, Khozin-Goldberg I, Boussiba S, et al. Lipid and fatty acid composition of the green oleaginous alga Parietochloris incisa, the richest plant source of arachidonic acid[J]. Phytochemistry, 2002, 60(5): 497−503. doi: 10.1016/S0031-9422(02)00100-0
[8]	Sanina N M, Goncharova S N, Kostetsky E Y. Fatty acid composition of individual polar lipid classes from marine macrophytes[J]. Phytochemistry, 2004, 65(6): 721−730. doi: 10.1016/j.phytochem.2004.01.013
[9]	Martin P, Van Mooy B A S, Heithoff A, et al. Phosphorus supply drives rapid turnover of membrane phospholipids in the diatom Thalassiosira pseudonana[J]. The ISME Journal, 2010, 5(6): 1057−1060.
[10]	Van Mooy B A S, Fredricks H F, Pedler B E, et al. Phytoplankton in the ocean use non-phosphorus lipids in response to phosphorus scarcity[J]. Nature, 2009, 458(7234): 69−72. doi: 10.1038/nature07659
[11]	Vogel G, Eichenberger W. Betaine lipids in lower plants. Biosynthesis of DGTS and DGTA in Ochromonas danica (Chrysophyceae) and the possible role of DGTS in lipid metabolism[J]. Plant and Cell Physiology, 1992, 33(4): 427−436.
[12]	Eichenberger W, Gfeller H, Grey P, et al. Gas chromatographic-mass spectrometric identification of betaine lipids in Chroomonas salina[J]. Phytochemistry, 1996, 42(4): 967−972. doi: 10.1016/0031-9422(96)00055-6
[13]	Abida H, Dolch L J, Meï C, et al. Membrane Glycerolipid remodeling triggered by nitrogen and phosphorus starvation in Phaeodactylum tricornutum[J]. Plant Physiology, 2015, 167(1): 118−136. doi: 10.1104/pp.114.252395
[14]	Roche S A, Leblond J D. Betaine lipids in chlorarachniophytes[J]. Phycological Research, 2010, 58(4): 298−305. doi: 10.1111/j.1440-1835.2010.00590.x
[15]	Cañavate J P, Armada I, Ríos J L, et al. Exploring occurrence and molecular diversity of betaine lipids across taxonomy of marine microalgae[J]. Phytochemistry, 2016, 124: 68−78. doi: 10.1016/j.phytochem.2016.02.007
[16]	Kröger N, Poulsen N. Diatoms-from cell wall biogenesis to nanotechnology[J]. Annual Review of Genetics, 2008, 42: 83−107. doi: 10.1146/annurev.genet.41.110306.130109
[17]	Li Yanrong, Lou Yamin, Mu Tong, et al. Simultaneous structural identification of diacylglyceryl-N-trimethylhomoserine (DGTS) and diacylglycerylhydroxymethyl-N, N, N-trimethyl-β-alanine (DGTA) in microalgae using dual Li⁺/H⁺ adduct ion mode by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry[J]. Rapid Communications in Mass Spectrometry, 2017, 31(5): 457−468. doi: 10.1002/rcm.7818
[18]	Kato M, Sakai M, Adachi K, et al. Distribution of betaine lipids in marine algae[J]. Phytochemistry, 1996, 42(5): 1341−1345. doi: 10.1016/0031-9422(96)00115-X
[19]	Flaim G, Obertegger U, Anesi A, et al. Temperature-induced changes in lipid biomarkers and mycosporine-like amino acids in the psychrophilic dinoflagellate Peridinium aciculiferum[J]. Freshwater Biology, 2014, 59(5): 985−997. doi: 10.1111/fwb.12321
[20]	Anesi A, Guella G. A fast liquid chromatography-mass spectrometry methodology for membrane lipid profiling through hydrophilic interaction liquid chromatography[J]. Journal of Chromatography A, 2015, 1384: 44−52. doi: 10.1016/j.chroma.2015.01.035
[21]	Van Mooy B A S, Fredricks H F. Bacterial and eukaryotic intact polar lipids in the eastern subtropical South Pacific: water-column distribution, planktonic sources, and fatty acid composition[J]. Geochimica et Cosmochimica Acta, 2010, 74(22): 6499−6516. doi: 10.1016/j.gca.2010.08.026
[22]	Haigh W G, Yoder T F, Ericson L, et al. The characterisation and cyclic production of a highly unsaturated homoserine lipid in Chlorella minutissima[J]. Biochimica et Biophysica Acta (BBA)—Lipids and Lipid Metabolism, 1996, 1299(2): 183−190. doi: 10.1016/0005-2760(95)00205-7
[23]	Galloway A W E, Britton-Simmons K H, Duggins D O, et al. Fatty acid signatures differentiate marine macrophytes at ordinal and family ranks[J]. Journal of Phycology, 2012, 48(4): 956−965. doi: 10.1111/j.1529-8817.2012.01173.x
[24]	Dalsgaard J, John M S, Kattner G, et al. Fatty acid trophic markers in the pelagic marine environment[J]. Advances in Marine Biology, 2003, 46: 225−340. doi: 10.1016/S0065-2881(03)46005-7
[25]	Thomas F, Ladislav H, Imke L, et al. Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection[J]. BMC Plant Biology, 2011, 11(1): 124. doi: 10.1186/1471-2229-11-124
[26]	Popendorf K J, Tanaka T, Pujo-Pay M, et al. Gradients in intact polar diacylglycerolipids across the Mediterranean Sea are related to phosphate availability[J]. Biogeosciences, 2011, 8(12): 3733−3745. doi: 10.5194/bg-8-3733-2011
[27]	Brandsma J, Hopmans E C, Brussaard C P D, et al. Spatial distribution of intact polar lipids in North Sea surface waters: relationship with environmental conditions and microbial community composition[J]. Limnology and Oceanography, 2013, 57(4): 959−973.