Molecular phylogenetic relationships of 40 species of Epinephelinae based on the partial sequences of 16S rRNA and COI genes

Liang Rishen; Chen Ming; Liao Guowei; Zhang Zhuowei; Zhang Guixin; Chen Yizhi; Lin Li

doi:10.3969/j.issn.0253-4193.2020.06.002

Volume 42 Issue 6

Nov. 2020

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Liang Rishen，Chen Ming，Liao Guowei, et al. Molecular phylogenetic relationships of 40 species of Epinephelinae based on the partial sequences of 16S rRNA and COI genes[J]. Haiyang Xuebao，2020, 42(6)：9–19 doi: 10.3969/j.issn.0253-4193.2020.06.002

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Molecular phylogenetic relationships of 40 species of Epinephelinae based on the partial sequences of 16S rRNA and COI genes

doi: 10.3969/j.issn.0253-4193.2020.06.002

Liang Rishen^{1, 2, 3
,},
Chen Ming¹,
Liao Guowei¹,
Zhang Zhuowei¹,
Zhang Guixin¹,
Chen Yizhi⁴,
Lin Li^{1, 2, 3
,
,}

1.
College of Animal Science and Technology, Zhongai University of Agriculture and Engineering, Guangzhou 510225, China
2.
Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou 510225, China
3.
Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225, China
4.
Shenzhen Levi Fresh Technology Development Co., Ltd., Shenzhen 518052, China

Received Date: 2019-09-11
Rev Recd Date: 2019-12-02

Available Online: 2020-11-18

Publish Date: 2020-06-25

Abstract

Abstract

In this study, 40 groupers belonging to 10 genera of the subfamily Epinephelinae from Western Pacific were collected, and partial sequences of 16S rRNA gene and COI gene were amplified and determined. The molecular phylogenetic relationships were constructed and analyzed using maximum likelihood method. The results showed that the length of COI gene was 651 bp, encoding 227 amino acids. While the consensus sequences of 16S rRNA gene were 566 bp, with certain base insertion and deletion. The sequences of 16S rRNA gene were more conserved than those of COI gene. The phylogenetic tree of the 10 genera of the subfamily Epinephelinae was constructed and the results showed that Plectropomus was first separated and rooted at the base of the tree, indicating its evolutionary status was most primitive. 6 species from Plectropomus were clustered as a monophyletic group. Aethaloperca was close to Cephalopholis, which tightly clustered together. Variola was located between Plectropomus and Cephalopholis. Epinephelus was located at the top of the tree, indicating its advanced evolutionary status. The species from Epinephelus in the tree were clustered as two parallel branches, instead of a monophyletic group; Cromileptes, Triso, Hyporthodus, Anyperodon and Promicrops could not form an independent branch but cluster together with species from Epinephelus, indicating their close relationship within Epinephelus and some species might belong to Epinephelus.
- Epinephelinae,
- 16S rRNA gene,
- COI gene,
- Phylogeny

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References(30)

References

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