Oxidative stress and energy utilization responses of juvenile cobia (<i>Rachycentron canadum</i>) to environmental hypoxia stress

Li Hongjuan; Chen Gang; Guo Zhixiong; Wang Weizheng; Huang Jiansheng; Zeng Zeqian

doi:10.3969/j.issn.0253-4193.2020.04.002

Volume 42 Issue 4

Nov. 2020

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Li Hongjuan，Chen Gang，Guo Zhixiong, et al. Oxidative stress and energy utilization responses of juvenile cobia ( Rachycentron canadum) to environmental hypoxia stress[J]. Haiyang Xuebao，2020, 42(4)：12–19，doi:10.3969/j.issn.0253−4193.2020.04.002

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Li Hongjuan，Chen Gang，Guo Zhixiong, et al. Oxidative stress and energy utilization responses of juvenile cobia ( Rachycentron canadum ) to environmental hypoxia stress[J]. Haiyang Xuebao，2020, 42(4)：12–19，doi:10.3969/j.issn.0253−4193.2020.04.002

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Oxidative stress and energy utilization responses of juvenile cobia (Rachycentron canadum) to environmental hypoxia stress

doi: 10.3969/j.issn.0253-4193.2020.04.002

College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China

Received Date: 2019-06-28
Rev Recd Date: 2019-10-17

Available Online: 2020-11-18

Publish Date: 2020-04-25

Abstract

Abstract

This study investigated the oxidative stress and energy utilization responses of juvenile cobia (Rachycentron canadum) to environmental hypoxia stress, and provided reference for the healthy cultivation of cobia. Through the hypoxia stress-reoxygenation test, the oxidative stress and energy utilization indexes of liver and muscle tissues were measured after hypoxia ((2.64±0.25)mg/L) stress for 3 h and reoxygenation ((6.34±0.15)mg/L) stress for 8 h, 24 h and 48 h. The results showed that after hypoxia stress, the activity of malondialdehyde (MDA), catalase (CAT) and glutathione reductase (GR) in the liver were significantly lower than that in the control group (p<0.05), and the activity of lactate dehydrogenase (LDH) was significantly higher than that in the control group (p<0.05). MDA and lipid peroxidase (LPO) activities in muscle were significantly lower than those in control group (p<0.05), and superoxide dismutase (SOD) and LDH activities were significantly higher than those in control group (p<0.05). The contents of muscle glycogen and liver glycogen were significantly lower than those of control group (p<0.01). During reoxygenation, the contents of MDA, LPO, SOD, CAT, glutathione peroxidase (GPx) and GR in liver and muscle all increased differentially. Liver glycogen content was significantly higher than that of the control group 24 h after reoxygenation (p<0.05), and significantly lower than that of the control group 48 h after reoxygenation (p<0.05). Muscle glycogen content was significantly lower than that of control group after reoxygenation for 8 h, 24 h and 48 h (p<0.05). In conclusion, hypoxia stress can cause some oxidative damage to the body of cobia, and the enzyme activity and energy supply of liver and muscle tissues change. The reoxygenation environment after hypoxia stress causes more severe oxidative damage to the body, which can be gradually restored to the normal level through physiological regulation.
- Rachycentron canadum,
- hypoxia stress,
- oxidative stress,
- energy utilization

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