Self-assembled membrane injection mass spectrometry system  and its application on the study of dissimilatory nitrate reduction in sandy sediments

Xie Chengjun; Song Guodong; Liu Sumei; Tang Jiyao; Zhang Guiling

doi:10.3969/j.issn.0253-4193.2020.02.003

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Xie Chengjun，Song Guodong，Liu Sumei, et al. Self-assembled membrane injection mass spectrometry system and its application on the study of dissimilatory nitrate reduction in sandy sediments[J]. Haiyang Xuebao，2020, 42(2)：22–29，doi:10.3969/j.issn.0253−4193.2020.02.003

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Self-assembled membrane injection mass spectrometry system and its application on the study of dissimilatory nitrate reduction in sandy sediments

doi: 10.3969/j.issn.0253-4193.2020.02.003

Xie Chengjun^{1, 2, 3
,},
Song Guodong^{1, 2
,
,},
Liu Sumei^{1, 2},
Tang Jiyao^{1, 2, 3},
Zhang Guiling^{1, 2}

1.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education/Institute for Advanced Ocean Study, Ocean University of China, Qingdao 266100, China
2.
Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
3.
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China

Received Date: 2019-04-05
Rev Recd Date: 2019-05-30

Available Online: 2020-11-18

Publish Date: 2020-02-25

Abstract

Abstract

Dissimilatory nitrate reduction processes in sediments play a crucial role in marine nitrogen cycle. The most popular method to determine the rates of different dissimilatory nitrate reduction processes is the ¹⁵N labeled technique. Therefore, accurate and rapid determination the concentration of ¹⁵N-labeled products, such as ²⁹N₂ and ³⁰N₂, is the key to quantify the rate of each dissimilatory nitrate reduction process. In this study, we set up a membrane injection mass spectrometry (MIMS) and optimize the operating condition of the MIMS for the determination of ²⁹N₂ and ³⁰N₂. The optimization experiment results indicated that, when the peristaltic pump for sampling flow rate is 0.80 mL/min, the sampling time is 3～3.5 min, the thermostat water bath temperature is 20～25℃, and the copper reduction furnace temperature is 300～600℃, the precision (expressed in coefficient of variation) of the measured ²⁹N₂/²⁸N₂ and ³⁰N₂/²⁸N₂ can be controlled less than 0.1% and 1%, respectively. We used the self-assembled MIMS and combined the ¹⁵N labeling technique to study the dissimilatory nitrate reduction processes in the sandy sediment of the Shilaoren beach in Qingdao. There is no significant aerobic denitrification in the Shilaoren sand that can completely reduce nitrate to N₂. The potential rates of anammox, anaerobic denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are (0.05±0.01) nmol/(cm³·h), (2.32±0.21) nmol/(cm³·h) and (1.02±0.15) nmol/(cm³·h) (N, wet sed.), respectively. Anaerobic denitrification is the major contributor to nitrate dissimilatory reduction, with a ratio of nearly 70%, followed by DNRA, with a ratio of up to 30%, while anammox has the lowest contribution of only 1%. In the N₂ production, the main contributor is anaerobic denitrification, and the contribution of anammox is only 2%.
- membrane injection mass spectrometry (MIMS),
- sandy sediment,
- denitrification,
- anammox,
- dissimilatory nitrate reduction to ammonium

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References(31)

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