Response of catalase in Antarctic ice alga <i>Chlamydomonas</i> sp. ICE-L to heat stress

Yuan Baosheng; Zhao Xia; Liu Chenlin; Zhang Peiyu

doi:10.3969/j.issn.0253-4193.2019.09.007

Volume 41 Issue 9

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Yuan Baosheng，Zhao Xia，Liu Chenlin, et al. Response of catalase in Antarctic ice alga Chlamydomonas sp. ICE-L to heat stress[J]. Haiyang Xuebao，2019, 41(9)：80–85，doi:10.3969/j.issn.0253−4193.2019.09.007

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Yuan Baosheng，Zhao Xia，Liu Chenlin, et al. Response of catalase in Antarctic ice alga Chlamydomonas sp. ICE-L to heat stress[J]. Haiyang Xuebao，2019, 41(9)：80–85，doi:10.3969/ j.issn.0253−4193.2019.09.007

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Response of catalase in Antarctic ice alga Chlamydomonas sp. ICE-L to heat stress

doi: 10.3969/j.issn.0253-4193.2019.09.007

1.
School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China
2.
Center for Marine Ecology Research, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China

Received Date: 2018-11-02
Rev Recd Date: 2019-04-09

Available Online: 2021-04-21

Publish Date: 2019-09-25

Abstract

Abstract

To investigate the heat stress responding strategies of Antarctic ice algae, the characteristics of a catalase gene CiCAT from the transcriptome of Antarctic ice alga Chlamydomonas sp. ICE-L were analyzed. The length of CiCAT is 2 066 bp encoding a catalase of 492 amino acids. In the phylogenetic tree of catalase amino acid sequences, Antarctic ice alga is clustered with green algae. The amino acid sequence identities of CiCAT are about 80.5% and 78.9% to the catalase from Dunaliella salina and Haematococcus lacustris, respectively. The changes of CiCAT gene expression and catalase activity in Antarctic ice alga were also investigated. Under heat stress, both the relative expression state of CiCAT gene and catalase activity changed from up-regulation to down-regulation over time. After heat stress treatment for 24 h, the expression of CiCAT gene was almost unchanged, while the enzyme activity in the heat treatment group was significantly higher than that in the control group. After 72 h's heat stress treatment, both gene expression and enzyme activity reached the highest level. Our preliminary results show that antioxidase system plays an important role in Antarctic ice algae responding to heat stress, which is similar to that in temperate algae and higher plants.
- Antarctic ice alga,
- heat stress,
- catalase

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References(24)

References

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