On the introduction of 15N2 tracer in marine N2 fixation rate assay

Chen Shengbao; Zhang Run; Chen Nengwang; Lai Limin; Chen Min; Zheng Minfang; Qiu Yusheng

doi:10.3969/j.issn.0253-4193.2018.10.005

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Chen Shengbao, Zhang Run, Chen Nengwang, Lai Limin, Chen Min, Zheng Minfang, Qiu Yusheng. On the introduction of 15N2 tracer in marine N2 fixation rate assay[J]. Haiyang Xuebao, 2018, 40(10): 42-50. doi: 10.3969/j.issn.0253-4193.2018.10.005

Citation:

Chen Shengbao, Zhang Run, Chen Nengwang, Lai Limin, Chen Min, Zheng Minfang, Qiu Yusheng. On the introduction of ¹⁵N₂ tracer in marine N₂ fixation rate assay[J]. Haiyang Xuebao, 2018, 40(10): 42-50. doi: 10.3969/j.issn.0253-4193.2018.10.005

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On the introduction of ¹⁵N₂ tracer in marine N₂ fixation rate assay

doi: 10.3969/j.issn.0253-4193.2018.10.005

1.
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China
2.
College of the Environment and Ecology, Xiamen University, Xiamen 361102, China

Received Date: 2018-04-15
Rev Recd Date: 2018-06-29

Abstract

Abstract

N₂ fixation plays an essential role in marine biogeochemical cycles by supplying new N. Stable isotope tracer (¹⁵N₂) assay has been considered as the best approach for measuring in situ N₂ fixation rate. Introducing ¹⁵N₂ in an efficient and clean way, and precisely determining the isotopic enrichment level in the incubation system, is thus fundamental for obtaining true rates. In this study, we show that modified bubble treatment is a recommended practice in introducing ¹⁵N₂. It can create ¹⁵N atomic abundance of substrate N₂ in the incubation bottle higher than 10%. Such enrichment is twice higher when compared with gas pre-dissolution treatment. Moreover, modified bubble treatment is featured by much lowered risk of trace metal contamination and less time/labor consuming. We propose that the modified bubble method coupled to stable isotope mass spectrometry should be widely used in measuring N₂ fixation rate in aquatic environments.
- N₂ fixation rate,
- ¹⁵N₂,
- isotope tracer assay

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References(13)

References

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