Effects of the extracts from the filtrate of Phaeodactylum triconutum on Prorocentrum donghaiense and Dunaliella salina analyzed by different physiological parameters

Xue Qiaona; Wang Rui; Tan Liju; Wang Jiangtao

doi:10.3969/j.issn.0253-4193.2018.04.011

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Xue Qiaona, Wang Rui, Tan Liju, Wang Jiangtao. Effects of the extracts from the filtrate of Phaeodactylum triconutum on Prorocentrum donghaiense and Dunaliella salina analyzed by different physiological parameters[J]. Haiyang Xuebao, 2018, 40(4): 119-126. doi: 10.3969/j.issn.0253-4193.2018.04.011

Citation:

Xue Qiaona, Wang Rui, Tan Liju, Wang Jiangtao. Effects of the extracts from the filtrate of Phaeodactylum triconutum on Prorocentrum donghaiense and Dunaliella salina analyzed by different physiological parameters[J]. Haiyang Xuebao, 2018, 40(4): 119-126. doi: 10.3969/j.issn.0253-4193.2018.04.011

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Effects of the extracts from the filtrate of Phaeodactylum triconutum on Prorocentrum donghaiense and Dunaliella salina analyzed by different physiological parameters

doi: 10.3969/j.issn.0253-4193.2018.04.011

1.
Key Laboratory of Marine Chemistry Theory and Technology/Ministry of Education, Ocean University of China, Qingdao 266100, China
2.
Shenzhen Key Laboratory for the Sustainable Use of Marine Biodiversity, Research Centre for the Oceans and Human Health, Shenzhen Research Institute, City University of Hong Kong, Shenzhen 518057, China

Received Date: 2017-04-18
Rev Recd Date: 2018-01-15

Abstract

Abstract

To investigate allelopathic effects of extracts from the filtrate of Phaeodactylum triconutum on growth and physiology of bloom-forming Prorocentrum donghaiense and Dunaliella salina, the cell density, cell size, membrane integrity and esterase activity were determined by the flow cytometry during a 6 d exposure test in laboratory. The results suggested that the growth of P. donghaiense was significantly inhibited by ethyl acetate extracts from the filtrate of P. triconutum. With the ethyl acetate extracts treatments, the cell size increased and the part of the algae membrane integrity was damaged, at the same time esterase activity significantly increased within a short time and was inhibited subsequently. However, the growth of D. salina was not inhibited by extracts from the filtrate of P. triconutum significantly. Both the cell size and membrane integrity was not inhibited, compared with the control cultures, the extracts induced negative effects on the esterase activity after 6 days exposure. These results confirmed that P. donghaiense was more sensitive to the allelochemical contained in the ethyl acetate of P. triconutum. Many P. donghaiense cells rapidly died and disappeared which be caused by the special internal structure of cells that the allelochemicals could easily enter the P. donghaiense cells. Esterase activity was the most sensitive parameter to evaluate the influence of the P. triconutum allelochemical on P. donghaiense, a kind of emergency mode of biological self-protection under environmental pressure.
- flow cytometry,
- the extracts from the filtrate of Phaeodactylum triconutum,
- cell size,
- membrane integrity,
- esterase activity

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References(27)

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