Effects of fermented <i>Enteromorpha prolifera</i> on growth performance, non-specific immunity and digestive enzyme activity of pearl gentian grouper

Xu Anle; Li Zhongbao; Shangguan Jingbo; Hu Xiaowei; Huang Yongchun

doi:10.3969/j.issn.0253-4193.2018.04.009

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Xu Anle, Li Zhongbao, Shangguan Jingbo, Hu Xiaowei, Huang Yongchun. Effects of fermented Enteromorpha prolifera on growth performance, non-specific immunity and digestive enzyme activity of pearl gentian grouper[J]. Haiyang Xuebao, 2018, 40(4): 96-105. doi: 10.3969/j.issn.0253-4193.2018.04.009

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Xu Anle, Li Zhongbao, Shangguan Jingbo, Hu Xiaowei, Huang Yongchun. Effects of fermented Enteromorpha prolifera on growth performance, non-specific immunity and digestive enzyme activity of pearl gentian grouper[J]. Haiyang Xuebao, 2018, 40(4): 96-105. doi: 10.3969/j.issn.0253-4193.2018.04.009

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Effects of fermented Enteromorpha prolifera on growth performance, non-specific immunity and digestive enzyme activity of pearl gentian grouper

doi: 10.3969/j.issn.0253-4193.2018.04.009

Fisheries College, Jimei University, Xiamen 361021, China;Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen 361021, China

Received Date: 2017-08-14
Rev Recd Date: 2017-09-16

Abstract

Abstract

To evaluate the effect of fermentation Enteromorpha prolifera on growth performance, digestive enzyme activities and serum non-specific immunity, 6 diets adding 0%, 1%, 2%, 3%, 4%, 5% fermentation E. prolifera were respectively fed to pearl gentian grouper(Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀) (19.16±0.25) g for 28 d. The results showed that with the increasing of fermentation E. prolifera level,the weight gain ratio(WGR) and the specific growth ratio(SGR) in each group showed a trend of first rising then falling,while the feed conversion ratio(FCR)was on the contrary. The WGR,SGR and FCR showed significantly better than the control group at the content of 2%(P<0.05). Adding fermentation E. prolifera enhanced the activities of serum total Superoxide dismutase (T-SOD), Alkaline phosphatase (AKP) and acid phosphatase (ACP) significantly (P<0.05), but no significant differences were found in the activities of Lysozyme (LZM) and Polyphenol Oxidase (PO) between trials (P>0.05). With the addition of fermentation E. prolifera, the activity of amylase, lipase and trypsin were significantly increased (P<0.05), and the best level is 2%-3%. In conclusion, fermentation E. prolifera can obviously promote the growth performance, improved the activity of non-specific immunity enzyme in the serum and digestion enzyme in the gut, it is recommended the amount adding is about 2%.
- fermented Enteromorpha prolifera,
- pearl gentian grouper,
- growth,
- digestive enzyme activity,
- non-specific immunity

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References(42)

References

姚东瑞. 浒苔[M]. 北京:海洋出版社, 2011:1-20, 106-147. Yao Dongrui. Enteromorpha[M]. Beijing:China Ocean Press, 2011:1-20, 106-147.

李信书, 徐军田, 姚东瑞, 等. 富营养化与生长密度对绿潮藻浒苔暴发性生长机制的影响[J]. 水产学报, 2013, 37(8):1206-1212. Li Xinshu, Xu Tuntian, Yao Dongrui, et al. Effects of nitrogen and phosphorus on the growth, photosynthesis and pigments of Ulva prolifera[J]. Journal of Fisheries of China, 2013, 37(8):1206-1212.

Li Yinping, Cui Jiefen, Zhang Gaoli, et al. Optimization study on the hydrogen peroxide pretreatment and production of bioethanol from seaweed Ulva prolifera biomass[J]. Bioresource Technology, 2016, 214:144-149.

Carpenter E J, Capone D G. Nitrogen in the Marine Environment[M]. New York:Academic Press, 1983.

高嵩, 范士亮, 韩秀荣, 等. 浒苔绿潮与南黄海近岸海域水质的关系[J]. 中国环境科学, 2014, 34(1):213-218. Gao Song, Fan Shiliang, Han Xiurong, et al. Relations of Enteromorpha prolifera blooms with temperature, salinity, dissolved oxygen and pH in the Southern Yellow Sea[J]. China Environmental Science, 2014, 34(1):213-218.

Scanlan C M, Wilkinson M. The use of seaweeds in biocide toxicity testing. Part 1. The sensitivity of different stages in the life-history of Fucus, and of other algae, to certain biocides[J]. Marine Environmental Research, 1987, 21(1):11-29.

王春忠, 孙富林, 侯代云, 等. 基于浒苔暴发海水池塘的微生物生态特征研究[J]. 海洋学报, 2017, 39(4):107-116. Wang Chunzhong, Sun Fulin, Hou Daiyun, et al. Study on the microbial characteristics of seawater pond based on Enteromorpha bloom[J]. Haiyang Xuebao, 2017, 39(4):107-116.

黄志俊. 浒苔固体快速发酵过程研究及产品开发[D]. 青岛:中国海洋大学, 2015. Huang Zhijun. Study on the rapid solid fermentation of Enteromorpha prolifera and its product development[D]. Qingdao:Ocean University of China, 2015.

杨欢, 黎中宝, 李元跃, 等. 厦门海域浒苔种类鉴定及其营养成分分析[J]. 中国渔业质量与标准, 2013, 3(4):70-75. Yang Huan, Li Zhongbao, Li Yuanyue, et al. Species identification and nutritional composition analysis of Enteromorpha in Xiamen coastal waters[J]. China Fishery Quality and Standard, 2013, 3(4):70-75.

张勇, 刘鹏霞, 程祥圣. 浒苔的利用和研究进展[J]. 海洋开发与管理, 2009, 26(8):97-100. Zhang Yong, Liu Pengxia, Cheng Xiangsheng. Progress in the usage and researches of Enteromorpha[J]. Ocean Development and Management, 2009, 26(8):97-100.

单俊伟, 刘海燕, 马栋. 浒苔的研究与资源化利用进展[J]. 现代农业科技, 2016(15):258-260. Shan Junwei, Liu Haiyan, Ma Dong. Research and resourcfulization application progress of Enteromorpha[J]. Modern Agricultural Science and Technology, 2016(15):258-260.

陈超, 孔祥迪, 李炎璐, 等. 棕点石斑鱼(♀)×鞍带石斑鱼(♂)杂交子代胚胎及仔稚幼鱼发育的跟踪观察[J]. 渔业科学进展, 2014, 35(5):135-144. Chen Chao, Kong Xiangdi, Li Yanlu, et al. Embryonic and morphological development in the larva, juvenile, and young stages of Epinephelus fuscoguttatus (♀)×E. lanceolatus(♂)[J]. Progress in Fishery Sciences, 2014, 35(5):135-144.

Yıldırım Ö, Ergün S, Yaman S, et al. Effects of two seaweeds (Ulva lactuca and Enteromorpha linza) as a feed additive in diets on growth performance, feed utilization, and body composition of rainbow trout (Oncorhynchus mykiss)[J]. Kafkas Vniversitesi Veteriner Fakültesi Dergisi, 2009, 15(3):455-460.

Asino H, Ai Qinghui, Mai Kangsen. Evaluation of Enteromorpha prolifera as a feed component in large yellow croaker (Pseudosciaena crocea, Richardson, 1846) diets[J]. Aquaculture Research, 2011, 42(4):525-533.

卢青, 杨宁, 王正丽, 等. 饲料中添加浒苔对大菱鲆生长和非特异性免疫力的影响[J]. 青岛农业大学学报:自然科学版, 2015, 32(1):62-66. Lu Qing, Yang Ning, Wang Zhengli, et al. The effects of dietary Entermorpha prolifera on the growth and non-specific immunity of juvenile turbot (Scophthalmus maximus)[J]. Journal of Qingdao Agricultural University:Natural Science, 2015, 32(1):62-66.

王万骞, 陈小金, 刘洋. 超微浒苔对凡纳滨对虾消化酶活力的影响[J]. 食品安全质量检测学报, 2015, 6(2):472-477. Wang Wanqian, Chen Xiaojin, Liu Yang. Effect of ultramicro Enteromorpha prolifera on activity of digestive enzymes in Litopenaeus vannamei[J]. Journal of Food Safety and Quality, 2015, 6(2):472-477.

高瞻, 陈强, 黎中宝, 等. 饲料添加发酵浒苔对花鲈(Lateolabrax japonicus)生长性能、消化酶活性和免疫的影响[J]. 海洋与湖沼, 2015, 46(6):1549-1556. Gao Zhan, Chen Qiang, Li Zhongbao, et al. Effect of fermentation Enteromorpha prolifera on growth performance, digestive enzyme activities and serum non-specific immunity of Lateolabrax japonicus[J]. Oceanologia et Limnologia Sinica, 2015, 46(6):1549-1556.

黎中宝. 饲料添加剂[M]. 厦门:厦门大学出版社, 2004:253-253. Li Zhongbao. Feed Additives[M]. Xiamen:Xiamen University Press, 2004:253-253.

钟礼云. 浒苔系列产品生理功能活性研究[D]. 福州:福建医科大学, 2008. Zhong Liyun. Studies on the serial products of Entermorpha with its activity and physiology functions[D]. Fuzhou:Fujian Medical University, 2008.

闫杰, 张欣, 孔晓静, 等. 饵料中浒苔添加量对梭鱼生长影响效果研究[J]. 饲料研究, 2012(3):64-65, 71. Yan Jie, Zhang Xin, Kong Xiaojing, et al. Research for the effects of dietary with Enteromorpha on the growth of Barracuda[J]. Feed Research, 2012(3):64-65, 71.

Yousif O M, Osman M F, Anawhi A R, et al. Growth response and carcass composition of rabbitfish, Siganus canaliculatus (Park) fed diets supplemented with dehydrated seaweed, Enteromorpha sp.[J]. Emirates Journal of Food and Agriculture, 2004, 16(2):18-26.

孙建风, 赵军, 张治国, 等. 饲粮中不同浒苔添加水平对肉鸡生长和屠宰性能的影响[J]. 畜牧与兽医, 2010, 42(7):33-36. Sun Jianfeng, Zhao Jun, Zhang Zhiguo, et al. Effect of different dietary levels of Enteromorpha proliferain on the growth and slaughter performance of Broiler chicken[J]. Animal Husbandry & Veterinary Medicine, 2010, 42(7):33-36.

胡静, 朱亚骏, 朱风华, 等. 浒苔对生长育肥猪消化能及养分消化率的影响[J]. 中国畜牧杂志, 2015, 51(3):29-32. Hu Jing, Zhu Yajun, Zhu Fenghua, et al. Effects of Enteromorpha prolifera on digestive energy and nutrient digestibility and in growing-finishing swine[J]. Chinese Journal of Animal Science, 2015, 51(3):29-32.

周蔚, 徐小明, 嵇珍, 等. 浒苔用作肉兔饲料的研究[J]. 江苏农业科学, 2001(6):68-69. Zhou Wei, Xu Xiaoming, Ji Zhen, et al. Research of dietary Enteromorpha prolifera on the meat rabbit[J]. Jiangsu Agricultural Sciences, 2001(6):68-69.

杨春花, 方希修. 海藻发酵饲料对樱桃谷肉鸭生长性能的影响[J]. 黑龙江畜牧兽医, 2012(4):81-82. Yang Chunhua, Fang Xixiu. Effect of seaweed fermentation feed on growth performance of cherry valley meat duck[J]. Heilongjiang Animal Science and Veterinary Medicine, 2012(4):81-82.

Rickert D A, Meyer M A, Hu J, et al. Effect of extraction pH and temperature on isoflavone and saponin partitioning and profile during soy protein isolate production[J]. Journal of Food Science, 2004, 69(8):C623-C631.

李晓, 王颖, 吴志宏, 等. 浒苔对刺参幼参生长影响的初步研究[J]. 中国水产科学, 2013, 20(5):1092-1099. Li Xiao, Wang Ying, Wu Zhihong, et al. Effect of Enteromorpha prolifera on growth of Apostichopus japohicus[J]. Journal of Fishery Sciences of China, 2013, 20(5):1092-1099.

秦搏, 常青, 陈四清, 等. 饲料中浒苔添加量以及处理方法对幼刺参生长、消化率、消化酶和非特异性免疫酶的影响[J]. 水产学报, 2015, 39(4):547-556. Qin Bo, Chang Qing, Chen Shiqing, et al. Effects of dosage and treatments of Enteromorpha prolifera on growth, digestibility, digestive enzymes and non-specific immunity enzymes of juvenile sea cucumber (Apostichopus japonicus Selenka)[J]. Journal of Fisheries of China, 2015, 39(4):547-556.

徐安乐, 蒋鹏达, 黎中宝, 等. 超微粉碎浒苔(Enteromorpha prolifera)对花鲈生长、非特异性免疫及消化酶活性的影响[J]. 海洋与湖沼, 2015, 46(6):1494-1501. Xu Anle, Jiang Pengda, Li Zhongbao, et al. Effects of Enteromorpha prolifera ultrafine powders on growth performance, non-specific immunity and digestive enzyme activity of Lateolabrax japonicus[J]. Oceanologia et Limnologia Sinica, 2015, 46(6):1494-1501.

李莉, 李春梅. 鱼类非特异性免疫研究进展[J]. 河南农业科学, 2012, 41(2):26-32. Li Li, Li Chunmei. Research progress on non-specific immune of fish[J]. Journal of Henan Agricultural Science, 2012, 41(2):26-32.

杨宁, 郭中帅, 王正丽. 饲料中添加浒苔对仿刺参幼参生长、消化酶活性和免疫力的影响[J]. 水产科学, 2016, 35(5):498-503. Yang Ning, Guo Zhongshuai, Wang Zhengli. Effect of dietary green laver Enteromorpha prolifera on growth, activity of digestive enzymes and immunity in sea cucumber (Apostichopus japonicus)[J]. Fisheries Science, 2016, 35(5):498-503.

Ajila C M, Brar S K, Verma M, et al. Solid-state fermentation of apple pomace using Phanerocheate chrysosporium-Liberation and extraction of phenolic antioxidants[J]. Food Chemistry, 2011, 126(3):1071-1080.

Wei Jianteng, Wang Shuxian, Liu Ge, et al. Polysaccharides from Enteromorpha prolifera enhance the immunity of normal mice[J]. International Journal of Biological Macromolecules, 2014, 64:1-5.

韩学凤, 张志标, 林伯坤, 等. 浒苔多糖研究进展[J]. 汕头大学学报:自然科学版, 2014, 29(3):46-50, 59. Han Xuefeng, Zhang Zhibiao, Lin Bokun, et al. The study of Enteromorpha polysaccharides[J]. Journal of Shantou University:Natural Science, 2014, 29(3):46-50, 59.

Umemura M, Kim J H, Aoyama H, et al. The iron chelating agent, deferoxamine detoxifies Fe(Salen)-induced cytotoxicity[J]. Journal of Pharmaceutical Sciences, 2017, 134(4):203-210.

Campa-Córdova A I, Hernández-Saavedra N Y, Ascencio F. Superoxide dismutase as modulator of immune function in American white shrimp (Litopenaeus vannamei)[J]. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 2002, 133(4):557-565.

Hur S J, Lee S Y, Kim Y C, et al. Effect of fermentation on the antioxidant activity in plant-based foods[J] Food Chemistry, 2014, 160:346-356.

Zhang Zuofa, Lv Guoying, Pan Huijuan, et al. Production of powerful antioxidant supplements via solid-state fermentation of wheat (Triticum aestivum Linn.) by cordyceps militaris[J]. Food Technology and Biotechnology, 2012, 50(1):32-39.

Cahu C, Rønnestad I, Grangier V, et al. Expression and activities of pancreatic enzymes in developing sea bass larvae (Dicentrarchus labrax) in relation to intact and hydrolyzed dietary protein involvement of cholecystokinin[J]. Aquaculture, 2004, 238(1/4):295-308.

郭娜. 不同饲料对刺参(Apostichopus japonicus)生长、消化生理和能量收支的影响[D]. 青岛:中国海洋大学, 2011. Guo Na. Studies on the effects of different diets on growth, digestive physiology and energy budget of Apostichopus japonicas[D]. Qingdao:Ocean University of China, 2011.

朱新产, 王宝维, 张庭荣. 海藻多糖对肉鸡生长性能影响的研究[J]. 动物营养学报, 2002, 14(4):54-58. Zhu Xinchan, Wang Baowei, Zhang Tingrong. Studies on effect of A. nodosum polysaccharides on growth of broilers[J]. Chinese Journal of Animal Nutrition, 2002, 14(4):54-58.

辛跃强. 低聚半乳糖对肠道益生菌作用机理的研究[D]. 济南:齐鲁工业大学, 2015. Xin Yueqiang. Research on the mechanism of GOS applied to intestinal probiotics[D]. Ji'nan:Qilu University of Technology, 2015.

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