Community composition and functional differences of symbiotic bacteria in healthy and blenching coral

Lin Zijun; Cai Zhonghua; Lin Guanghui; Zhou Jin

doi:10.3969/j.issn.0253-4193.2018.02.011

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Lin Zijun, Cai Zhonghua, Lin Guanghui, Zhou Jin. Community composition and functional differences of symbiotic bacteria in healthy and blenching coral[J]. Haiyang Xuebao, 2018, 40(2): 104-116. doi: 10.3969/j.issn.0253-4193.2018.02.011

Citation:

Lin Zijun, Cai Zhonghua, Lin Guanghui, Zhou Jin. Community composition and functional differences of symbiotic bacteria in healthy and blenching coral[J]. Haiyang Xuebao, 2018, 40(2): 104-116. doi: 10.3969/j.issn.0253-4193.2018.02.011

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Community composition and functional differences of symbiotic bacteria in healthy and blenching coral

doi: 10.3969/j.issn.0253-4193.2018.02.011

1.
Center for Earth System Science, Tsinghua University, Beijing 100084, China
2.
Division of Ocean Science and Technology Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
3.
Center for Earth System Science, Tsinghua University, Beijing 100084, China;Division of Ocean Science and Technology Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China

Received Date: 2017-07-15

Abstract

Abstract

In order to assess the influence of bleaching on coral (Pocillopora damicornis) symbiotic microbial communities and functions, microbial structure and functional diversity were evaluated using OTU analysis and BIOLOG Ecoplates method. The results showed that Chao Ⅰ index was significantly lower in bleaching individuals than the healthy ones (P<0.05). Among the microbial communities (genus level), Synechococcus was the dominant population (more than 50%) in the experimental groups and the controls, and not found the significant difference between the two status. However, some significant differences have observed. Pelagibacter, Puniceispirillum, Coraliomargarita and Photobacterium were abundant in healthy individuals, whereas Actinomarina, Blastopirellula and some pathogenic bacteria(like Vibrio, Pseudospirillum, Alteromonas and Coxiella)were enrichment in bleaching coral. Personal analysis shows that the temperature and nutrients (nitrate and phosphate) were the most correlated environmental factors with the species composition. Functional analysis using average well color development (AWCD) showed that the order of carbon source utilization in healthy group was carboxylic acids > amino acids > amphiphiles carbohydrate > polymers > amine. Whereas the sugars, amino acids and amines were the preferred carbon sources in bleaching group. The different utilization ability of carboxylic acid and sugar result in carbon metabolism change between the two groups. To the nitrogen source, both of the groups dominated by inorganic nitrogen, but the bleaching group showed certain ability to utilize organic peptide. Inorganic phosphorus was the main phosphorus source in both groups and it has the priority to be use. Compared with the healthy groups, the bleaching individuals decrease some ability to consumption organic polyphosphates. Taken together, this work showed that the bleaching event reduced coral symbiotic microbial diversity and changed the metabolic potential to C, N, and P source. Bleaching event had a significant impact on coral symbiotic microbial communities and functional activity.
- coral,
- bleaching,
- symbiotic bacteria,
- structural composition,
- functional differences

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