Selection and characterization of an improved strain(<i>A</i>-18)by hybridization recombinant in <i>Pyropia yezoensis</i>(Bangiales,Rhodophyta)

Jiang Hao; Ding Hongchang; Yan Xinghong

doi:10.3969/j.issn.0253-4193.2018.02.010

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Jiang Hao, Ding Hongchang, Yan Xinghong. Selection and characterization of an improved strain(A-18)by hybridization recombinant in Pyropia yezoensis(Bangiales,Rhodophyta)[J]. Haiyang Xuebao, 2018, 40(2): 95-103. doi: 10.3969/j.issn.0253-4193.2018.02.010

Citation:

Jiang Hao, Ding Hongchang, Yan Xinghong. Selection and characterization of an improved strain(A-18)by hybridization recombinant in Pyropia yezoensis(Bangiales,Rhodophyta)[J]. Haiyang Xuebao, 2018, 40(2): 95-103. doi: 10.3969/j.issn.0253-4193.2018.02.010

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Selection and characterization of an improved strain(A-18)by hybridization recombinant in Pyropia yezoensis(Bangiales,Rhodophyta)

doi: 10.3969/j.issn.0253-4193.2018.02.010

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China

Received Date: 2017-07-10

Abstract

Abstract

In order to select a new variety of Pyropia yezoensis with fast growing and wild-type color,following intraspecific hybridization of the green-type pigmentation mutant and red-type pigmentation in P. yezoensis was carried out,and then an improved strain (A-18) was isolated from the recombinants. The mean length,mean length-width ratio and wet weight of 60-day-old blades in A-18 strain were 84.95 cm,49.46 and 0.52 g,which were 3.12,7.01 and 1.36 times those of WT strain. After being cultured for 60 days,the contents of chlorophyll a and phycobiliprotein in A-18 strain were 8.37 and 53.81 mg/g,respectively,both of them were close to the WT strain. The thickness of 60-day-old blades of A-18 strain was 20.22 μm,which was 29% thinner than that of WT strain. In addition,the total number of conchospores released from A-18 strain was 916.01×10⁴ per shell,which was 1.55 times that of WT strain. The above results showed that A-18 strain was characterized by fast growth,large length-width raito,large amount of conchospores,and its color is close to the wild-type,therefore,A-18 strain is expected to be applied in the production.
- Pyropia yezoensis,
- intraspecific hybridization,
- improved strain,
- gametophytic blade,
- growth rate,
- thickness

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References(27)

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