Cloning, spatiotemporal expression and SNPs identification of <i>GRB</i>2 gene in <i>Sinonovacula constricta</i>

Zhao Jiaxi; Cui Baoyue; Dong Yinghui; Yao Hanhan; Lin Zhihua

doi:10.3969/j.issn.0253-4193.2018.02.009

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Zhao Jiaxi, Cui Baoyue, Dong Yinghui, Yao Hanhan, Lin Zhihua. Cloning, spatiotemporal expression and SNPs identification of GRB2 gene in Sinonovacula constricta[J]. Haiyang Xuebao, 2018, 40(2): 87-94. doi: 10.3969/j.issn.0253-4193.2018.02.009

Citation:

Zhao Jiaxi, Cui Baoyue, Dong Yinghui, Yao Hanhan, Lin Zhihua. Cloning, spatiotemporal expression and SNPs identification of GRB2 gene in Sinonovacula constricta[J]. Haiyang Xuebao, 2018, 40(2): 87-94. doi: 10.3969/j.issn.0253-4193.2018.02.009

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Cloning, spatiotemporal expression and SNPs identification of GRB2 gene in Sinonovacula constricta

doi: 10.3969/j.issn.0253-4193.2018.02.009

1.
Key Laboratory of Aquatic Germplasm Resource of Zhejiang Province, Zhejiang Wanli University, Ningbo 315100, China
2.
College of Fishery and Life Sciences, Shanghai Ocean University, Shanghai 201306, China

Received Date: 2017-07-11

Abstract

Abstract

To explore the molecular structure and biological function of GRB2 in Sinonovacula constricta(Sc-GRB2), its cDNA was cloned by SMART RACE techniques, then the bioinformatics, SNPs in exon and expression profiles in different tissues and developmental stages were analyzed. The results indicated that the full length cDNA of Sc-GRB2 gene was 1 223 bp, containing a complete 711 bp ORF encoding 236 amino acids. Comparisons of animal acid sequence, Sc-GRB2 has high homology with bivalves of Meretrix meretrix and Tegillarca granosa (64% and 59%). Sc-GRB2 has homologous with others share 45%-58% similarity, it indicate GRB2 is relatively conservative. The result of qRT-PCR in different tissues showed that Sc-GRB2 expressed in all seven tissues, the expression of foot was extremely significantly higher than others (P<0.01). The relative expression in different stages revealed that the expression of juvenile clams was extremely significantly higher than other stages (P<0.01). A total of 11 SNPs in the exon of Sc-GRB2 were identified.
- Sinonovacula constricta,
- GRB2,
- gene cloning,
- SNP

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References(34)

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