Effects of dietary Chitosan oligosaccharide on the performance, digestion and serum biochemical indexes of the juvenile Japanese seabass (<i>Lateolabrax japonicus</i>)

Hu Xiaowei; Shangguan Jingbo; Li Zhongbao; Yang Min; Xu Anle

doi:10.3969/j.issn.0253-4193.2018.02.007

Article Contents

Article Navigation > Haiyang Xuebao > 2018 > 40(2): 69-76

Hu Xiaowei, Shangguan Jingbo, Li Zhongbao, Yang Min, Xu Anle. Effects of dietary Chitosan oligosaccharide on the performance, digestion and serum biochemical indexes of the juvenile Japanese seabass (Lateolabrax japonicus)[J]. Haiyang Xuebao, 2018, 40(2): 69-76. doi: 10.3969/j.issn.0253-4193.2018.02.007

Citation:

Hu Xiaowei, Shangguan Jingbo, Li Zhongbao, Yang Min, Xu Anle. Effects of dietary Chitosan oligosaccharide on the performance, digestion and serum biochemical indexes of the juvenile Japanese seabass (Lateolabrax japonicus)[J]. Haiyang Xuebao, 2018, 40(2): 69-76. doi: 10.3969/j.issn.0253-4193.2018.02.007

Citation:

Hu Xiaowei, Shangguan Jingbo, Li Zhongbao, Yang Min, Xu Anle. Effects of dietary Chitosan oligosaccharide on the performance, digestion and serum biochemical indexes of the juvenile Japanese seabass (Lateolabrax japonicus)[J]. Haiyang Xuebao, 2018, 40(2): 69-76. doi: 10.3969/j.issn.0253-4193.2018.02.007

PDF( 922 KB)

Effects of dietary Chitosan oligosaccharide on the performance, digestion and serum biochemical indexes of the juvenile Japanese seabass (Lateolabrax japonicus)

doi: 10.3969/j.issn.0253-4193.2018.02.007

Fisheries College, Jimei University, Xiamen 361021, China;Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen 361021, China

Received Date: 2017-07-15

Abstract

Abstract

This research was conducted to evaluate the effects of dietary supplementation with Chitosan-oligosaccharide (COS) on the growth performance, digestion, serum biochemical indexes and intestinal flora. A total of 360 juvenile Japanese seabass were randomly assigned to six treatment diets that included COS at concentrations of 0%,0.2%,0.4%,0.6%,0.8%,1.0% for 45 d. Each treatment had 3 replicates with 20. The results showed:(1) The growth performance:The weight gain and specific growth rate of Diet3(0.6%) and Diet4(0.8%) were increased compared with the control group (P>0.05); (2) The digestive ferment:The intestinal trypsin activity were significantly increased (P<0.05) in the Diet3(0.6%), Diet4(0.8%) and Diet5(1.0%). Intestinal lipase activity was remarkably increased at Diet2(0.4%),Diet3(0.6%) and Diet4(0.8%) (P<0.05). There was no significant difference in intestinal amylase activity (P>0.05). (3) The serum biochemical indexes:Compared with the control group, the contents of the serum total protein and high-density lipoprotein cholesterol (HDL-C) was obviously increased (P<0.05); (4) The nonspecific immunity:The contents of MDA was decreased significantly (P>0.05) on the Diet3(0.6%). The activities of superoxide dismutase, alkaline phosphatase and peroxidase were all increased (P>0.05) on the Diet1 (0.2%), and the serum lysozyme was significantly increased (P<0.05). (5) Intestinal flora:The number of Salmonella in Diet3 (0.6%), Diet 4(0.8%) and Diet5(1%), and E. coli was significantly decreased (P<0.05) in Diet(0.8%). The quantity of bifidobacteria increased significantly (P<0.05) in Diet1(0.2%), Diet2(0.4%) and Diet3(0.6%). In conclusion, these results indicate that COS can enhance growth and immunity of juvenile Japanese seabass,and the optimum addition level was 0.6%~0.8% in diet.
- Lateolabrax japonicas,
- chitosan-oligosaccharide,
- growth performance,
- non-specific immune,
- intestinal flora

FullText(HTML)

References(29)

References

Swiatkiewicz S, Swiatkiewicz M, Arczewska-Wlosek A, et al. Chitosan and its oligosaccharide derivatives (Chito-oligosaccharides) as feed supplements in poultry and swine nutrition[J]. Journal of Animal Physiology and Animal Nutrition, 2015, 99(1):1-12.

陈海燕, 张彬, 何勇松. 壳寡糖的研究进展和应用前景[J]. 广东畜牧兽医科技, 2007, 32(2):17-20, 29. Chen Haiyan, Zhang Bin, He Yongsong. Research progress and application foreground of chitosan oligosaccharide[J]. Guangdong Journal of Animal and Veterinary Science, 2007, 32(2):17-20, 29.

黄鑫玮, 杨莎莎, 刘毅, 等. 壳寡糖对幼建鲤生长性能、脂肪代谢、非特异性免疫功能和肠道健康的影响[J]. 动物营养学报, 2015, 27(7):2106-2114. Huang Xinwei Yang Shasha Liu Yi, et al. Effect of dietary chitosan on growth performance, lipid metabolism, non-specific immune function and intestinal health of juvenile Jian carp (Cyprinus carpio Var. Jian)[J]. Chinese Journal of Animal Nutrition, 2015, 27(7):2106-2114.

蔡胜昌, 张利民, 张德瑞, 等. 壳寡糖与低聚木糖对大菱鲆(Scophthalmus maximus)幼鱼生长、体组成和血液生化指标的影响[J]. 渔业科学进展, 2015, 36(6):29-36. Cai Shengchang, Zhang Limin, Zhang Derui, et al. Effects of chitosan oligosaccharide and Xylo-oligosaccharide on the growth performance, body composition and serum biochemistry of juvenile turbots (Scophthalmus maximus)[J]. Progress in Fishery Sciences, 2015, 36(6):29-36.

沈锦玉, 刘问, 曹铮, 等. 免疫增强剂对中华绒螯蟹免疫功能的影响[J]. 浙江农业学报, 2004, 16(1):25-29. Shen Jinyu, Liu wen, Cao Zheng, et al. Effect of immunity-stimulants on immunity function of Eriocheir sinensis[J]. Acta Agriculturae Zhejiangensis, 2004, 16(1):25-29.

潘金露. 饲料中壳寡糖和褐藻酸寡糖对大菱鲆(Scophthalmus maximus)消化及肠道菌群的影响[D]. 大连:大连海洋大学, 2016. Pan Jinlu. Effect of chitosan oligosaccharide and alginate oligosaccharide on digestive ability and gastrointestinal microbial community of juvenile turbot (Scophthalmus maximus)[D]. Dalian:Dalian Ocean University, 2016.

Refstie S, Storebakken T, Roem A J. Feed consumption and conversion in Atlantic salmon (Salmo salar) fed diets with fish meal, extracted soybean meal or soybean meal with reduced content of oligosaccharides, trypsin inhibitors, lectins and soya antigens[J]. Aquaculture, 1998, 162(3/4):301-312.

Lin Shimei, Mao Shuhong, Guan Yong, et al. Dietary administration of chitooligosaccharides to enhance growth, innate immune response and disease resistance of Trachinotus ovatus[J]. Fish & Shellfish Immunology, 2012, 32(5):909-913.

刘含亮, 孙敏敏, 王红卫, 等. 壳寡糖对虹鳟生长性能、血清生化指标及非特异性免疫功能的影响[J]. 动物营养学报, 2012, 24(3):479-486. Liu Hanliang, Sun Minmin, Wang Hongwei, et al. Effects of Chito-oligosaccharides on growth performance, serum biochemical indices and non-specific immunity function of rainbow trout (Oncorhynchus mykiss)[J]. Chinese Journal of Animal Nutrition, 2012, 24(3):479-486.

孙立威, 文华, 蒋明, 等. 壳寡糖对吉富罗非鱼幼鱼生长性能、非特异性免疫及血液学指标的影响[J]. 广东海洋大学学报, 2011, 31(3):43-49. Sun Liwei, Wen Hua, Jiang Ming, et al. Effect of dietary chitosan on growth performance, non-specific immunity and hematological parameters of juvenile GIFT Tilapia, Oreochromis niloticus[J]. Journal of Guangdong Ocean University, 2011, 31(3):43-49.

张晓萍, 勇强, 余世袁. 青春双歧杆菌体外代谢低聚木糖的研究[J]. 南京林业大学学报(自然科学版), 2010, 34(1):5-8. Zhang Xiaoping, Yong Qiang, Yu Shiyuan. In vitro fermentation of xylooligosaccharides by Bifidobacterium adolescentis[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2010, 34(1):5-8.

王子花, 申瑞玲, 李文全. 短链脂肪酸的产生及作用[J]. 畜牧兽医科技信息, 2007(2):12-13. Wang Zihua, Shen Ruiling, Li Wenquan. The production and function of short chain fatty acids[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine, 2007(2):12-13.

Zafar T A, Weaver C M, Zhao Yongdong, et al. Nondigestible oligosaccharides increase calcium absorption and suppress bone resorption in ovariectomized rats[J]. The Journal of Nutrition, 2004, 134(2):399-402.

Jung W K, Moon S H, Kim S K. Effect of chitooligosaccharides on calcium bioavailability and bone strength in ovariectomized rats[J]. Life Sciences, 2006, 78(9):970-976.

黄纪明, 白树民, 朱德兵, 等. 低聚异麦芽糖对模拟失重大鼠肠道益生菌以及钙代谢和骨矿盐密度影响的初步研究[J]. 中国微生态学杂志, 2002, 14(4):189-191. Huang Jiming, Bai Shumin, Zhu Debing, et al. The effects of isomaltooligosaccharide on the gut probiotics and femur's BMD and calcium metabolism in simulating weightlessness rats[J]. Chinese Journal of Microecology, 2002, 14(4):189-191.

谭崇桂, 冷向军, 李小勤, 等. 多糖、寡糖、蛋白酶对凡纳滨对虾生长、消化酶活性及血清非特异性免疫的影响[J]. 上海海洋大学学报, 2013, 22(1):93-99. Tan Chonggui, Leng Xiangjun, Li Xiaoqin, et al. Effects of polysaccharides, oligosaccharides and protease on growth, digestive enzyme activities and serum nonspecific immunity of white shrimp (Litopenaeus vannamei)[J]. Journal of Shanghai Ocean University, 2013, 22(1):93-99.

Sang H M, Fotedar R, Filer K. Effects of dietary mannan oligosaccharide on the survival, growth, immunity and digestive enzyme activity of freshwater crayfish, Cherax destructor Clark (1936)[J]. Aquaculture Nutrition, 2011, 17(2):e629-e635.

郑炫禹, 田熙英, 徐大方, 等. 含有壳寡糖的淀粉酶活性抑制用组合物[P]. 中国:CN104884066A, 2015-09-02. Zheng Xuanyu, Tian Xiying, Xu Dafang, et al. Amylase-activity-inhibiting composition containing Chito-oligosaccharide[P]. CN:CN104884066A, 2015-09-02.

Li X J, Piao X S, Kim S W, et al. Effects of Chito-oligosaccharide supplementation on performance, nutrient digestibility, and serum composition in broiler chickens[J]. Poultry Science, 2007, 86(6):1107-1114.

刘冰, 刘万顺, 韩宝芹, 等. 壳寡糖及其衍生物对实验性糖尿病大鼠调节血脂和抗氧化作用[J]. 山东大学学报:理学版, 2006, 41(4):158-163. Liu Bing, Liu Wanshun, Han Baoqin, et al. Effect of chitooligosaccharides and its derivatives on regulating plasma lipid and improving antioxidant ability in diabetic mouse induced by STZ[J]. Journal of Shandong University, 2006, 41(4):158-163.

White W B, Bird H R, Sunde M L, et al. Viscosity of β-D-glucan as a factor in the enzymatic improvement of barley for chicks[J]. Poultry Science, 1983, 62(5):853-862.

Magnadóttir B, Crispin M, Royle L, et al. The carbohydrate moiety of serum IgM from Atlantic cod (Gadus morhua L.)[J]. Fish & Shellfish Immunology, 2002, 12(3):209-227.

Yang H S, Xiong X, Li J Z, et al. Effects of Chito-oligosaccharide on intestinal mucosal amino acid profiles and alkaline phosphatase activities, and serum biochemical variables in weaned piglets[J]. Livestock Science, 2016, 190:141-146.

常青, 梁萌青, 王家林, 等. 壳聚糖对花鲈生长和非特异性免疫力的影响[J]. 海洋水产研究, 2006, 27(5):17-22. Chang Qing, Liang Mengqing, Wang Jialin, et al. Influence of chitosan on the growth and non-specific immunity of Japanese sea bass (Lateolabrax japonicus)[J]. Marine Fisheries Research, 2006, 27(5):17-22.

Lin Shimei, Mao Shuhong, Guan Yong, et al. Effects of dietary chitosan oligosaccharides and Bacillus coagulans on the growth, innate immunity and resistance of koi (Cyprinus carpio koi)[J]. Aquaculture, 2012, 342-343:36-41.

Winston G W. Oxidants and antioxidants in aquatic animals[J]. Comparative Biochemistry and Physiology Part C:Comparative Pharmacology, 1991, 100(1/2):173-176.

李阳, 常文环, 张姝, 等. 饲粮添加壳寡糖和干酪乳杆菌对肉鸡生长性能、肌肉品质及抗氧化性能的影响[J]. 动物营养学报, 2016, 28(5):1450-1461. Li Yang, Chang Wenhuan, Zhang Shu, et al. Effects of dietary chitosan oligosaccharide and Lactobacillus casei on growth performance, meat quality and antioxidant function of broilers[J]. Chinese Journal of Animal Nutrition, 2016, 28(5):1450-1461.

Helander I M, Nurmiaho-Lassila E L, Ahvenainen R, et al. Chitosan disrupts the barrier properties of the outer membrane of Gram-negative bacteria[J]. International Journal of Food Microbiology, 2001, 71(2/3):235-244.

徐后国, 艾庆辉, 麦康森, 等. 饲料中添加枯草芽孢杆菌和壳寡糖对大黄鱼幼鱼血清免疫指标的影响[J]. 中国海洋大学学报, 2011, 41(7/8):42-47. Xu Houguo, Ai Qinghui, Mai Kangsen, et al. Effects of dietary administration of Bacillus subtilis and Chito-oligosaccharide on serum immune parameters of juvenile large croaker, Pseudosciaena crocea[J]. Periodical of Ocean University of China, 2011, 41(7/8):42-47.

Relative Articles

Supplements(0)

Cited By

Proportional views