Microscopic observation of the reproductive development of gametophyte and tetrasporohyte of <i>Gracilariopsis lemaneiformis</i> (Rhodophyta)

Mi Ping; Sui Zhenghong; Que Zhou; Zhou Wei; Shang Erlei

doi:10.3969/j.issn.0253-4193.2017.08.010

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Mi Ping, Sui Zhenghong, Que Zhou, Zhou Wei, Shang Erlei. Microscopic observation of the reproductive development of gametophyte and tetrasporohyte of Gracilariopsis lemaneiformis (Rhodophyta)[J]. Haiyang Xuebao, 2017, 39(8): 99-114. doi: 10.3969/j.issn.0253-4193.2017.08.010

Citation:

Mi Ping, Sui Zhenghong, Que Zhou, Zhou Wei, Shang Erlei. Microscopic observation of the reproductive development of gametophyte and tetrasporohyte of Gracilariopsis lemaneiformis (Rhodophyta)[J]. Haiyang Xuebao, 2017, 39(8): 99-114. doi: 10.3969/j.issn.0253-4193.2017.08.010

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Microscopic observation of the reproductive development of gametophyte and tetrasporohyte of Gracilariopsis lemaneiformis (Rhodophyta)

doi: 10.3969/j.issn.0253-4193.2017.08.010

The Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Ministry of Education, Qingdao 266003, China

Received Date: 2016-10-28
Rev Recd Date: 2017-02-24

Abstract

Abstract

A series of cell morphology and structure changes were displayed during the development of reproductive structure of gametophyte and tetrasporaphyte of Gracilariopsis lemaneiformis. In this study, the gametophyte and different strains of tetrasporophyte were used to make continuous free hand section, in order to observe the development of the gametophyte and the formation of tetraspores in different strains of G. lemaneiformis. The result showed that the development of male and female gametophytes undertook seedling phage (SP), hair cell phage (HCP) and sexual mature phage (SMP). There was no significant difference between male and female gametophyte in SP and HCP. The male and female gametophyte were differentiable after entering SMP when the reproductive apparatus formed. The tetraspores were derived from the epidermal cells of tetrasprophyte, the initial tetrasporogonium was scarlet, and 5-10 μm in diameter. It later grew to 20-25 μm in diameter and became mature. The mature tetrasporogonium divided into four initial tetraspores gathered together, and then the tetraspores were released out of the matrix and became globular. During the development of tetraspores, the density of floridean starch granules decreased in medullary part. Cultivar 981 formed more deformed tetraspores, which may be one of the reasons leading to the low fertility of cultivar 981. The layers of epidermal cells, the numbers and the volume of medullary cells changed continuously from the basal to the tip part. Pit connections existed wildly in the epidermal, cortical and medullary cells, however varying by size, numbers and length between different layers. The released tetraspores were observed attaching on the tetrasporophyte, and the holdfast grew to cover the tetrasporophyte or invaded into the wound tissue of tetrasporophyte, which may cause gametophyte and tetrasporophyte generation promiscuous and lead to the genetic complexity of G. lemaneiformis.
- Gracilariopsis lemaneiformis,
- gametophyte,
- tetrasporophyte,
- reproductive apparatus,
- microscopic observation

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