The diversity changes of bacterial community in mariculture water of <i>Litopenasus vannamei</i> at Zhangpu, Fujian Province

Hu Dong; Wang Liping; Zhao Ran; Shao Zongze

doi:10.3969/j.issn.0253-4193.2017.08.009

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Hu Dong, Wang Liping, Zhao Ran, Shao Zongze. The diversity changes of bacterial community in mariculture water of Litopenasus vannamei at Zhangpu, Fujian Province[J]. Haiyang Xuebao, 2017, 39(8): 89-98. doi: 10.3969/j.issn.0253-4193.2017.08.009

Citation:

Hu Dong, Wang Liping, Zhao Ran, Shao Zongze. The diversity changes of bacterial community in mariculture water of Litopenasus vannamei at Zhangpu, Fujian Province[J]. Haiyang Xuebao, 2017, 39(8): 89-98. doi: 10.3969/j.issn.0253-4193.2017.08.009

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The diversity changes of bacterial community in mariculture water of Litopenasus vannamei at Zhangpu, Fujian Province

doi: 10.3969/j.issn.0253-4193.2017.08.009

1.
School of Public Health, Xiamen University, Xiamen 361005, China;Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanograph, State Oceanic Administration, Xiamen 361005, China
2.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanograph, State Oceanic Administration, Xiamen 361005, China
3.
School of Public Health, Xiamen University, Xiamen 361005, China

Received Date: 2017-01-18
Rev Recd Date: 2017-02-22

Abstract

Abstract

To explore the diversity changes of bacterial communities in mariculture water, water samples from Litopenasus vannamei culture ponds in the middle and late growth stages at the farms located at Zhangpu, Fujian Province, China, were collected. Bacterial communities were analyzed through Illumina Miseq sequencing based on 16S rRNA gene, and the water quality parameters were also detected. The results showed that the water quality factors, such as NH₄⁺-N, COD, fluctuated greatly compared with other parameters. 19 phyla, 35 classes, 80 orders, 135 families and 254 bacterial genera were detected,which indicated a high diversity of microbial flora. Proteobacteria and Bacteroidetes are the main dominant group during culture, and while there was a certain fluctuation of microbial composition in different aquaculture periods, no obvious change law was observed. Cyanobacteria showed high abundance at the day of 67 and 115, and their abundance varied greatly during culture (0.7% to 63.9%). In addition, the abundance of probiotics and potential pathogens is low during culture. The RDA results showed that COD/NO₃^- and COD contents in water had the greatest influence on bacterial communities, and reduced DO can increase bacterial richness. This study revealed the change of bacterial community in the middle and late stages of L. vannamei culture, and found that water quality factors had a significant effect on microbial composition. The results provided a significant reference for the healthy aquaculture of L. vannamei.
- Litopenasus vannamei,
- microbial community,
- 16S rRNA gene high throughput sequencing,
- water quality parameters

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References(31)

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