Salicylic acid-concentrated sulfuric acid colorimetry method for determination of nitrate content in <i>Ulva prolifera</i>

Zhao Qian; Shi Xiaoyong; Chen Yuehong; Wang Lisha; Tang Hongjie

doi:10.3969/j.issn.0253-4193.2017.02.011

Article Contents

Article Navigation > Haiyang Xuebao > 2017 > 39(2): 112-119

Zhao Qian, Shi Xiaoyong, Chen Yuehong, Wang Lisha, Tang Hongjie. Salicylic acid-concentrated sulfuric acid colorimetry method for determination of nitrate content in Ulva prolifera[J]. Haiyang Xuebao, 2017, 39(2): 112-119. doi: 10.3969/j.issn.0253-4193.2017.02.011

Citation:

Zhao Qian, Shi Xiaoyong, Chen Yuehong, Wang Lisha, Tang Hongjie. Salicylic acid-concentrated sulfuric acid colorimetry method for determination of nitrate content in Ulva prolifera[J]. Haiyang Xuebao, 2017, 39(2): 112-119. doi: 10.3969/j.issn.0253-4193.2017.02.011

Citation:

PDF( 1649 KB)

Salicylic acid-concentrated sulfuric acid colorimetry method for determination of nitrate content in Ulva prolifera

doi: 10.3969/j.issn.0253-4193.2017.02.011

1.
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China;Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China
2.
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China;Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China;National Marine Hazard Mitigation Service, Beijing 100194, China

Received Date: 2016-08-30
Rev Recd Date: 2016-11-04

Abstract

Abstract

Green-tide events occurred in the Yellow Sea successively in recent years. The marine macrophyte Ulva prolifera is the dominant green-tide-forming seaweed. It is necessary to study the nitrogen contents in the algal tissue, which contributes to knowing the uptake and assimilation of nitrate by Ulva prolifera. In this study, the method of salicylic acid-concentrated sulfuric acid colorimetry was employed to investigate the nitrate contents in Ulva prolifera. The influenced factors, including sample pretreatment, the amount of chromogenic reagent addition and the selection of performance curve, were examined and optimized during the determinations. The detection limit, precision and accuracy of this method were also established simultaneously. Our results indicated that this method was applicable for the fast examination of the nitrate contents in the algal tissue.
- Ulva prolifera,
- salicylic acid-concentrated sulfuric acid colorimetry,
- nitrate

FullText(HTML)

References(25)

References

Li Yan, Xiao Jie, Ding Lanping, et al. Community structure and controlled factor of attached green algae on the Porphyra yezoensis aquaculture rafts in the Subei Shoal, China[J]. Acta Oceanologica Sinica, 2015, 34(8):93-99.

丁兰平, 栾日孝. 浒苔(Enteromorpha prolifera)的分类鉴定、生境习性及分布[J]. 海洋与湖沼, 2009, 40(1):68-71. Ding Lanping, Luan Rixiao. The taxonomy, habit, and distribution of a green alga Enteromorpha prolifera (Ulvales, Chlorophyta)[J]. Oceanologia et Limnologia Sinica, 2009, 40(1):68-71.

Raffaelli D G, Raven J A, Poole L J. Ecological impact of green macroalgal blooms[J]. Oceanography & Marine Biology, 1998, 36:97-125.

Valiela I, McClelland J, Hauxwell J, et al. Macroalgal blooms in shallow estuaries:controls and ecophysiological and ecosystem consequences[J]. Limnology and Oceanography, 1997, 42(5 part 2):1105-1118.

Wu Lifen, Chen P C, Lee C M. The effects of nitrogen sources and temperature on cell growth and lipid accumulation of microalgae[J]. International Biodeterioration & Biodegradation, 2013, 85:506-510.

Li Ji, Glibert P M, Zhou Mingjiang. Temporal and spatial variability in nitrogen uptake kinetics during harmful dinoflagellate blooms in the East China Sea[J]. Harmful Algae, 2010, 9(6):531-539.

Grasshoff K, Ehrhardt M, Kremling K. Methods of Seawater Analysis[M]. 2nd ed. Wienhien:Verlag Chemie, 1983:180-182.

Dennis M J, Key P E, Papworth T, et al. The determination of nitrate and nitrite in cured meat by HPLC/UV[J]. Food Additives & Contaminants, 1990, 7(4):455-461.

Cheng C F, Tsang C W. Simultaneous determination of nitrite, nitrate and ascorbic acid in canned vegetable juices by reverse-phase ion-interaction HPLC[J]. Food Additives & Contaminants, 1998, 15(7):753-758.

Siu D C, Henshall A. Ion chromatographic determination of nitrate and nitrite in meat products[J]. Journal of Chromatography A, 1998, 804(1/2):157-160.

Zhou Meisu, Guo Donglong. Simultaneous determination of chloride, nitrate and sulphate in vegetable samples by single-column ion chromatography[J]. Microchemical Journal, 2000, 65(3):221-226.

Butt S B, Riaz M, Iqbal M Z. Simultaneous determination of nitrite and nitrate by normal phase ion-pair liquid chromatography[J]. Talanta, 2001, 55(4):789-797.

Andrade R, Viana C O, Guadagnin S G, et al. A flow-injection spectrophotometric method for nitrate and nitrite determination through nitric oxide generation[J]. Food Chemistry, 2003, 80(4):597-602.

Usher C D, Telling G M. Analysis of nitrate and nitrite in foodstuffs:a critical review[J]. Journal of the Science of Food and Agriculture, 1975, 26(11):1793-1805.

Ximenes M I N, Rath S, Reyes F G R. Polarographic determination of nitrate in vegetables[J]. Talanta, 2000, 51(1):49-56.

Pérez-Olmos R, Herrero R, Lima J L F C, et al. Sequential potentiometric determination of chloride and nitrate in meat products[J]. Food Chemistry, 1997, 59(2):305-311.

Öztekin N, Nutku M S, Erim F B. Simultaneous determination of nitrite and nitrate in meat products and vegetables by capillary electrophoresis[J]. Food Chemistry, 2002, 76(1):103-106.

国家环保局. 水和废水监测分析方法[M]. 北京:中国环境科学出版社, 1997:259-261. National Environmental Protection Agency. Water and Wastewater Monitoring and Analysis Methods[M]. Beijing:China Environmental Science Press, 1997:259-261.

于立红, 康立娟, 于晓斌, 等. 蔬菜中硝酸盐污染程度快速测定方法的研究[J]. 吉林农业大学学报, 2005, 27(1):76-78. Yu Lihong, Kang Lijuan, Yu Xiaobin, et al. Study on fast determination method of nitrite pollution in vegetable[J]. Journal of Jilin Agricultural University, 2005, 27(1):76-78.

Yang J E, Kim J J, Skogley E O, et al. A simple spectrophotometric determination of nitrate in water, resin, and soil extracts[J]. Soil Science Society of America Journal, 1998, 62(4):1108-1115.

李俭平. 浒苔对氮营养盐的响应及其氮营养盐吸收动力学和生理生态研究[D]. 青岛:中国科学院海洋研究所, 2011. Li Jianping. The response to nitrogen nutrient, and the uptake kinetics mechanism of nitrogen and Ecophysiological analysis of Enteromorpha prolifera[D]. Qingdao:Institute of Oceanology, Chinese Academy of Sciences, 2011.

叶静, 张喆, 李富超, 等. 大型绿藻浒苔转化表达系统选择标记的筛选[J]. 生物技术通报, 2006(3):63-67. Ye Jing, Zhang Zhe, Li Fuchang, et al. Screening of selectable marker for transformation and expression system of green seaweed Enteromorpha clathrata[J]. Biotechnology Bulletin, 2006(3):63-67.

张寒野, 吴望星, 宋丽珍, 等. 条浒苔海区试栽培及外界因子对藻体生长的影响[J]. 中国水产科学, 2006, 13(5):781-786. Zhang Hanye, Wu Wangxing, Song Lizhen, et al. Trial and field cultivation of Enteromorpha clathrata and effects of ecological factors on its growth[J]. Journal of Fishery Sciences of China, 2006, 13(5):781-786.

李帮秀, 张贺翠, 王三根, 等. 蔬菜硝酸盐含量测定方法的改进[J]. 植物生理学报, 2014, 50(11):1749-1752. Li Bangxiu, Zhang Hecui, Wang Sangen, et al. An improved testing method for determining nitrate contents in fresh vegetable[J]. Plant Physiology Journal, 2014, 50(11):1749-1752.

赵文宽, 贺飞, 方程, 等. 仪器分析[M]. 北京:高等教育出版社, 2000:5-6. Zhao Wenkuan, He Fei, Fang Cheng, et al. Instrument Analysis[M]. Beijing:Higher Education Press, 2000:5-6.

Relative Articles

Supplements(0)

Cited By

Proportional views