Identification and differential expression of gill microRNA in the Chinese surf clam (<i>Mactra chinensis</i>) with Cd<sup>2+</sup> exposure

Zhang Jingjing; Li Hongjun; Qin Yanjie; Liu Min; Ye Sheng

doi:10.3969/j.issn.0253-4193.2016.12.012

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Zhang Jingjing, Li Hongjun, Qin Yanjie, Liu Min, Ye Sheng. Identification and differential expression of gill microRNA in the Chinese surf clam (Mactra chinensis) with Cd2+ exposure[J]. Haiyang Xuebao, 2016, 38(12): 118-131. doi: 10.3969/j.issn.0253-4193.2016.12.012

Citation:

Zhang Jingjing, Li Hongjun, Qin Yanjie, Liu Min, Ye Sheng. Identification and differential expression of gill microRNA in the Chinese surf clam (Mactra chinensis) with Cd²⁺ exposure[J]. Haiyang Xuebao, 2016, 38(12): 118-131. doi: 10.3969/j.issn.0253-4193.2016.12.012

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Identification and differential expression of gill microRNA in the Chinese surf clam (Mactra chinensis) with Cd²⁺ exposure

doi: 10.3969/j.issn.0253-4193.2016.12.012

1.
Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, China;College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
2.
Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, China
3.
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China

Received Date: 2016-06-30

Abstract

Abstract

The Chinese surf clam (Mactra chinensis) is an economically important clam, distributed in Liaoning Province and Shandong Province. In recently years, because of coastal environmental deterioration and overfishing, the natural population of M. chinensis have considerably declined. In this paper, we studied the microRNA transcriptome of gills, and control and experimental group were sequenced through Illumina Hiseq 2500 CE. The differential expression andlysis was used to find the functional microRNA response to the Cd²⁺ exposure. Through Illumina Hi-seq 2500 CE, a total of 14 415 256 clean reads and 15 570 111 clean reads were yielded in the gill of control and experimental group respectively. A total of 14 584 077 small RNA, including 187 859 unique small RNA were yielded. The distribution of the small RNA length in the two library was similar, most of them were 26-27 nt. 27 nt was the most abundant length in control group, followded by 28 nt, 26 nt, and 23 nt; 26 nt was the most abundant length in experimental group, and followed by 27 nt, 28 nt and 23 nt. 50 microRNA was found in unique small RNA, including 38 conserved and 12 novel genes. The most abundant length of microRNA in the two library was the same, 23 nt. Through the analyze of differential expression analysis, the expression of 5 microRNA was induced with significantly difference, other 45 microRNA was regulated up or down without significantly difference. 542 target genes were yielded when the 50 microRNA were hit to mRNA genome. And the target genes of differential expression microRNA were annotated by hitting to the NCBI database, and 4 genes hit to the COG, 1 genes hit to the GO, 5 genes hit to the KEGG and 11 genes hit to the nr database. The genes hit to the NCBI database included E3 ubiquitin-protein ligase, Wnt signaling pathway and Regulator of G-protein signaling 22.
- Mactra chinensis,
- cadmium ion,
- microRNA,
- differential expression,
- functional genes

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References(43)

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