Diversity of microbe and hydrogenase genes from a coastal hot spring of Kalianda, Indonesia

He Peiqing; Dewi Seswita Zilda; Li Jiang; Zhang Xuelei; Cui Jingjing; Bai Yazhi; Gintung Patantis; Ekowati Chasanah

doi:10.3969/j.issn.0253-4193.2016.06.013

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He Peiqing, Dewi Seswita Zilda, Li Jiang, Zhang Xuelei, Cui Jingjing, Bai Yazhi, Gintung Patantis, Ekowati Chasanah. Diversity of microbe and hydrogenase genes from a coastal hot spring of Kalianda, Indonesia[J]. Haiyang Xuebao, 2016, 38(6): 119-129. doi: 10.3969/j.issn.0253-4193.2016.06.013

Citation:

He Peiqing, Dewi Seswita Zilda, Li Jiang, Zhang Xuelei, Cui Jingjing, Bai Yazhi, Gintung Patantis, Ekowati Chasanah. Diversity of microbe and hydrogenase genes from a coastal hot spring of Kalianda, Indonesia[J]. Haiyang Xuebao, 2016, 38(6): 119-129. doi: 10.3969/j.issn.0253-4193.2016.06.013

Citation:

He Peiqing, Dewi Seswita Zilda, Li Jiang, Zhang Xuelei, Cui Jingjing, Bai Yazhi, Gintung Patantis, Ekowati Chasanah. Diversity of microbe and hydrogenase genes from a coastal hot spring of Kalianda, Indonesia[J]. Haiyang Xuebao, 2016, 38(6): 119-129. doi: 10.3969/j.issn.0253-4193.2016.06.013

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Diversity of microbe and hydrogenase genes from a coastal hot spring of Kalianda, Indonesia

doi: 10.3969/j.issn.0253-4193.2016.06.013

1.
Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
2.
Research Center for Marine and Fisheries Product Processing and Biotechnology, Agency for Marine and Fisheries Research and Development, Ministry of Marine and Fisheries Affairs, Jakarta 40115, Indonesia
3.
Key laboratory of State Oceanic Administration for Marine Sedimentology & Environmental Geology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

Received Date: 2015-07-07

Abstract

Abstract

During July, 2011, samples were collected from a coastal hot spring of Kalianda, Indonesia. Clone libraries of 16S rRNA genes of bacteria and archaea, and hydrogenase genes were constructed. The sequences were determined, and the diversity were also analyzed. The results demonstrated that the coastal hot spring hosted 16 phyla. Proteobacteria was dominant in hydrothermal fluid and sediment, accounting for 59.5% and 73.3%, respectively. Cyanobacteria was the most abundant in mat with abundance of 56.5%. Ammonia oxidizing Crenarchaeota was predominant in archaea. More than 70% of the species were most related to marine microbes, indicating the marine property of this system. About 20% of the species were most related to the thermophilic or moderate thermophilic microbes from terrestrial hot springs. These species might originate from the hyperthermal subfloor and involve in the process of iron oxidation and reduction, hydrogen oxidation, sulfur oxidation and nitrate reduction. The composition and distribution of NAD(P)-linked bidirectional NiFe-hydrogenase and FeFe-hydrogenase genes were also shaped by temperature and salinity. Our research provided an insight into the shallow-sea hydrothermal system.
- Kalianda Indonesia,
- coastal hot spring,
- 16S rRNA genes,
- hydrogenase

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References(33)

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