Cloning and expression analysis of a C-type lectin gene (Mm-Lec1) in hard clam <em>Meretrix meretrix</em>

Zhang Jingjing; Li Hongjun; Qin Yanjie; Liu Min; Ye Sheng

doi:10.3969/j.issn.0253-4193.2016.06.012

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Zhang Jingjing, Li Hongjun, Qin Yanjie, Liu Min, Ye Sheng. Cloning and expression analysis of a C-type lectin gene (Mm-Lec1) in hard clam Meretrix meretrix[J]. Haiyang Xuebao, 2016, 38(6): 110-118. doi: 10.3969/j.issn.0253-4193.2016.06.012

Citation:

Zhang Jingjing, Li Hongjun, Qin Yanjie, Liu Min, Ye Sheng. Cloning and expression analysis of a C-type lectin gene (Mm-Lec1) in hard clam Meretrix meretrix[J]. Haiyang Xuebao, 2016, 38(6): 110-118. doi: 10.3969/j.issn.0253-4193.2016.06.012

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Cloning and expression analysis of a C-type lectin gene (Mm-Lec1) in hard clam Meretrix meretrix

doi: 10.3969/j.issn.0253-4193.2016.06.012

1.
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, China
2.
Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, China
3.
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China

Received Date: 2015-10-15

Abstract

Abstract

The hard clam (Meretrix meretrix) is an economically important bivalve species in China. Aquaculture of M. meretrix is seriously affected by the epidemic diseases. The study of the immune system of M. meretrix is an important approach to solve disease problems. C-type lectins (CTLs) play important roles in the identification of pathogen associated molecular patterns and activation of humoral immunity. In the present study, a C-type lectin gene of M. meretrix(denoted as Mm-Lec1) was obtained through sequencing full-length cDNA library. The full-length cDNA of Mm-Lec1 was 586 bp with a 486 bp open reading frame, encoding 161 amino acids. The length of 5' and 3' untranslated region was 21 and 79 bp, respectively. The molecular weight of predicted protein was 18.65 kD, and the theoretical isoelectric point was 4.98. The predicted amino acid sequence had a signal peptide (Met1-Ser19), a sugar-binding site (QPN) and a carbohydrate recognition domain (CRD). The tertiary structure of Mm-Lec1 was predicted as a compact type and had a β sheet structure with three beta sheet layers. The similarity between Mm-Lec1 and the other species mentioned in this study were 20%-32%. Mm-Lec1, Mytilus galloprovincialis CTL 6 and Chlamys farreri CTL A were clustered in one branch in the neighbor-joining (NJ) tree. Mm-Lec1 mRNA was expressed in all tested tissues, including gill, haemocytes, hepatopancreas, mantle, adductor muscle and gonad, with the highest expression level in gill, the second in haemocytes, and the least in gonad. In the bacteria exposure test, the mRNA expression level was lowest at 6 h, and highest at 48 h, suggesting that Mm-Lec1 play a role in defensing against bacterial invasion in M. meretrix.
- Meretrix meretrix,
- C-type lectin,
- EST,
- tertiary structure,
- real-time PCR

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References(31)

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