The amplification and FISH analysis of 5S rDNA in <em>Liobagrus marginatus</em>

Zhang Weiwei; Fu Yuanshuai; Shao Yan; Wang Baoqin; Shi Dongli; Zhang Lan

doi:10.3969/j.issn.0253-4193.2016.06.009

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Article Navigation > Haiyang Xuebao > 2016 > 38(6): 82-88

Zhang Weiwei, Fu Yuanshuai, Shao Yan, Wang Baoqin, Shi Dongli, Zhang Lan. The amplification and FISH analysis of 5S rDNA in Liobagrus marginatus[J]. Haiyang Xuebao, 2016, 38(6): 82-88. doi: 10.3969/j.issn.0253-4193.2016.06.009

Citation:

Zhang Weiwei, Fu Yuanshuai, Shao Yan, Wang Baoqin, Shi Dongli, Zhang Lan. The amplification and FISH analysis of 5S rDNA in Liobagrus marginatus[J]. Haiyang Xuebao, 2016, 38(6): 82-88. doi: 10.3969/j.issn.0253-4193.2016.06.009

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The amplification and FISH analysis of 5S rDNA in Liobagrus marginatus

doi: 10.3969/j.issn.0253-4193.2016.06.009

1.
Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, Binzhou University, Binzhou 256603, China
2.
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

Received Date: 2015-06-03

Abstract

Abstract

In present research,5S rDNA was amplified by the usual 5S rDNA primers in Genomic DNA of female and male Liobagrus marginatus,and sequenced. The results of amplification showed that there were not different in 5S rDNA between female and male of L.marginatus,and length of 5S rDNA was 117 bp. Using the method of double color Fluorescence In situ Hybridization (FISH),5S rDNA and 45S rDNA on metaphase chromosomes were located in L.marginatus. The results showed that 5S rDNA and 45S rDNA were located on sex chromosome and the 5th chromosome,respectively. Phylogenetic tree of 5S rDNA in L.marginatus and 22 fishes else was constructed by DNAman,so evolution position of L.marginatus in fishes was studied preliminarily. This study offer data accumulating and basis of analysis,to illustrate phyletic evolution of fishes and repeat sequences on sex chromosome,and relation of heterochromatin or repeat sequences and sex determination & differentiation in vertebrates.
- Liobagrus marginatus,
- PCR,
- 5S rDNA,
- phylogenetic tree,
- chromosome,
- FISH

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References(20)

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