Genetic analysis of common venomous Cubozoa and Scyphozoa in Thailand waters

Liu Ruijuan; Xiao Jie; Zhang Xuelei; Charatsee Aungtonya

doi:10.3969/j.issn.0253-4193.2016.06.006

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Liu Ruijuan, Xiao Jie, Zhang Xuelei, Charatsee Aungtonya. Genetic analysis of common venomous Cubozoa and Scyphozoa in Thailand waters[J]. Haiyang Xuebao, 2016, 38(6): 51-61. doi: 10.3969/j.issn.0253-4193.2016.06.006

Citation:

Liu Ruijuan, Xiao Jie, Zhang Xuelei, Charatsee Aungtonya. Genetic analysis of common venomous Cubozoa and Scyphozoa in Thailand waters[J]. Haiyang Xuebao, 2016, 38(6): 51-61. doi: 10.3969/j.issn.0253-4193.2016.06.006

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Genetic analysis of common venomous Cubozoa and Scyphozoa in Thailand waters

doi: 10.3969/j.issn.0253-4193.2016.06.006

1.
The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;Key Laboratory of Science and Engineering for Marine Ecology and Environment, State Oceanic Administration, Qingdao 266061, China
2.
Phuket Marine Biological Center, Phuket 83000, Thailand

Received Date: 2015-11-16

Abstract

Abstract

The genetic diversity of common venomous Cubozoa and Scyphozoa jellyfish in the coastal waters of Thailand was investigated using the nuclear 18S and mitochondrial 16S rDNA genes. It was also compared about the capability of these two molecular markers to discriminate those common jellyfish species. There were 9 genetically distinctive species among the 32 samples collected from Thai waters, including 4 Cubozoa species, 4 Scyphozoans and 1 hydrozoan. However, it needs further information on the morphology and life cycle to determine the true taxonomic status of most species in this research. The K2P (Kimura 2-parameter) genetic distance was evaluated within and among the species based on the sequences of these two genes. The results showed much higher genetic divergence based on 16S gene (0－0.050 and 0.204－0.474 for intra-and inter-species genetic distances, respectively) compared with those using 18S gene (0－0.002 and 0.008－0.066 for intra-and inter-species genetic distances, respectively). At the same time, the A and T nucleotide composition of 16S gene was obviously higher than 18S gene. And significant variation of nucleotide composition was detected between two jellyfish classes using 16S gene, but not 18S gene. These results further indicated the relatively higher mutation rate of 16S gene, which could be applied for the genetic diversity studies among jellyfish species with close genetic relationship and the intra-species genetic population studies.
- Scyphozoa,
- Cubozoa,
- genetic diversity,
- ribosomal RNA gene (rDNA),
- Thailand

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