Study on antibiotic resistance and resistant related genes of <i>Escherichia coli</i> from seawater of bathing beach

Wang Yue; Su Jie; Ming Hongxia; Shi Yan; Zhao Sha; Guan Daoming; Fan Jingfeng

doi:10.3969/j.issn.0253-4193.2015.12.013

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Wang Yue, Su Jie, Ming Hongxia, Shi Yan, Zhao Sha, Guan Daoming, Fan Jingfeng. Study on antibiotic resistance and resistant related genes of Escherichia coli from seawater of bathing beach[J]. Haiyang Xuebao, 2015, 37(12): 123-128. doi: 10.3969/j.issn.0253-4193.2015.12.013

Citation:

Wang Yue, Su Jie, Ming Hongxia, Shi Yan, Zhao Sha, Guan Daoming, Fan Jingfeng. Study on antibiotic resistance and resistant related genes of Escherichia coli from seawater of bathing beach[J]. Haiyang Xuebao, 2015, 37(12): 123-128. doi: 10.3969/j.issn.0253-4193.2015.12.013

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Study on antibiotic resistance and resistant related genes of Escherichia coli from seawater of bathing beach

doi: 10.3969/j.issn.0253-4193.2015.12.013

1.
National Marine Environmental Monitoring Center, Dalian 116023, China;College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
2.
National Marine Environmental Monitoring Center, Dalian 116023, China;The Ecological Environment Key Laboratory of Coastal Waters of the State Oceanic Administration, Dalian 116023, China

Received Date: 2015-05-22
Rev Recd Date: 2015-09-27

Abstract

Abstract

To study antibiotic resistance pattern and resistant gene of Escherichia coli from seawater recreational beach,41E.coli isolates from seawater in Dalian Xinghai bathing beach were identified and characterized using biochemical tests. Antimicrobial resistance of E.coli isolates was determined by Kirby-Bauer disk diffusion method,the result showed highest resistance to tetracycline(46%) and lowest resistance against ammonia QuNa(5%). Multidrug resistance was observed in 34% of 41E.coli isolates. Only tetracycline resistant gene tetA and tetB gene were detected among the 41 isolates using PCR method. The detection frequency of tetA gene was 34% in genome DNA and 29% in plasmid DNA of E.coli. While tetB gene was detected in 2% of E.coli genome DNA and in 12% of E.coli plasmid DNA. χ²test suggest that tetracycline resistant phenotype of E.coli is correlated with tetracycline resistant gene.
- Escherichia coli,
- seawater of bathing beach,
- antibiotic resistance,
- resistant gene

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References(22)

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