Antibacterial activity and mechanisms of heterotetramer hemoglobin derived polypeptide from <i>Tegillarca granosa</i>

Wang Juanjuan; Bao Yongbo; Wang Sufang; Lin Zhihua

doi:10.3969/j.issn.0253-4193.2015.12.011

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Wang Juanjuan, Bao Yongbo, Wang Sufang, Lin Zhihua. Antibacterial activity and mechanisms of heterotetramer hemoglobin derived polypeptide from Tegillarca granosa[J]. Haiyang Xuebao, 2015, 37(12): 106-115. doi: 10.3969/j.issn.0253-4193.2015.12.011

Citation:

Wang Juanjuan, Bao Yongbo, Wang Sufang, Lin Zhihua. Antibacterial activity and mechanisms of heterotetramer hemoglobin derived polypeptide from Tegillarca granosa[J]. Haiyang Xuebao, 2015, 37(12): 106-115. doi: 10.3969/j.issn.0253-4193.2015.12.011

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Antibacterial activity and mechanisms of heterotetramer hemoglobin derived polypeptide from Tegillarca granosa

doi: 10.3969/j.issn.0253-4193.2015.12.011

1.
School of Marine Sciences, Ningbo University, Ningbo 315211, China;Zhejiang Collaborative Innovation Center of Ocean Efficient Healthy Culture, Ningbo 315211
2.
Zhejiang Key Laboratory of Aquatic Germplasm Resources, Zhejiang Wanli University, Ningbo 315100, China
3.
School of Marine Sciences, Ningbo University, Ningbo 315211, China;Zhejiang Key Laboratory of Aquatic Germplasm Resources, Zhejiang Wanli University, Ningbo 315100, China

Received Date: 2015-07-20
Rev Recd Date: 2015-09-28

Abstract

Abstract

Tg-HbⅡ(heterogeneous tetramer) and Tg-HbⅠ(homodimer) were purified by gel chromatography with a recovery 48.15% and 24.91%. Seven major peptides(F1-F7) were purified by reverse phase C-18 column from trysin enzymatic hydrolysates of Tg-HbⅡ. The agar diffusion method was used to detect their antibacterial activity. Results showed that all peptide collections exhibited no antibacterial activity to Staphylococcus aureus,Pseudomonas putida,Eschetichia coli,Bacillus firmus and B. subtilis; but peptides F2-F7 showed antibacterial activity against Vibrio alginolyticus,V. harveyi and V. parahaemolyticus. MICs of peptide F2-F7 toward V. alginolyticus,V. parahaemolyticus and V. harveyi were 0.012-0.200 mg/mL Using fluorescence microscopy to observe the cell that treated with peptide,found that vibrio cells die rapidly in 5 min. Using Transmission electron microscopy to observe V. alginolyticus and V. harveryi after antibacterial peptides treatment,the results showed that the peptides changed permeability of cytoplasmic membrane,leading to leaking out of cytoplasmic contents. Finally,the affected Vibrio died due to leakage of the cell contents. Research results showed that the Tegillarca granosa hemoglobin source of antimicrobial peptides have obvious antibacterial activity against vibrio and antimicrobial peptides kill bacteria by damaging its internal structure,these findings laid a solid foundation to the research of antibacterial mechanism of hemoglobin.
- Tegillarca granosa,
- enzymolysis peptides,
- RP-HPLC,
- antibacterial activity,
- ultrastructure,
- antibacterial mechanism

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References(21)

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