Isolation and characterization of an improved strain of <em>Pyropia dentata </em>(Bangiales, Rhodophyta) after being irradiated by <sup>60</sup>Co-γ ray

Li Shuping; Yan Xinghong

doi:10.3969/j.issn.0253-4193.2015.10.007

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Li Shuping, Yan Xinghong. Isolation and characterization of an improved strain of Pyropia dentata (Bangiales, Rhodophyta) after being irradiated by 60Co-γ ray[J]. Haiyang Xuebao, 2015, 37(10): 69-79. doi: 10.3969/j.issn.0253-4193.2015.10.007

Citation:

Li Shuping, Yan Xinghong. Isolation and characterization of an improved strain of Pyropia dentata (Bangiales, Rhodophyta) after being irradiated by ⁶⁰Co-γ ray[J]. Haiyang Xuebao, 2015, 37(10): 69-79. doi: 10.3969/j.issn.0253-4193.2015.10.007

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Isolation and characterization of an improved strain of Pyropia dentata (Bangiales, Rhodophyta) after being irradiated by ⁶⁰Co-γ ray

doi: 10.3969/j.issn.0253-4193.2015.10.007

Li Shuping¹,
Yan Xinghong^2
,

1.
College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China
2.
College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China

Received Date: 2015-04-29

Abstract

Abstract

The young gametophytic blades of Pyropia dentata, developed from conchospores of the wild-type strain (PD-WT), were treated with ⁶⁰Co-γ ray to induce mutation in the experiment. After being cultured for 4 weeks, there were many color-mutated cell clusters showing bright red, red brown, red orange and yellow green in the treated blades. The results showed that the percentage of the colored-mutated cell-clusters increased as increasing of the irradiation dose and from the base to the tip of the blades. Single color-mutated cells were isolated enzymatically from the color-mutated blades and were regenerated into blades. An improved strain named as PD-5 strain with the growth advantages was selected from the regenerated blades. In the growth of the blades, the maximum and average absolute growth rates of this improved strain were 3.76 cm/d and 2.71 cm/d, which were 3.60 and 4.22 times that of the PD-WT strain, respectively, during culture from 30 to 70 days. The mean length of F₁ gametophytic blades of PD-5 strain was 117.42 cm which was 4.32 times that of the PD-WT strain after being cultured for 70 days. The contents of Chl a and phycobiliprotein of thePD-5 strain were 8.41 mg/g and 97.07 mg/g, respectively, increasing by 25.71% and 104.44% in contrast with that of the PD-WT,respectively. The mean thickness of the 45-day-old blades of the PD-5 was 26.79 μm,decreasing by 35.04% in contrast with that of the PD-WT. The total numbers of the conchospores released from the PD- 5 strain was 421.16×10⁴ per shell,which was 2.19 times that of the PD-WT strain. The aboved results confirmed that the PD-5 strain was characterized by faster growth,higher contents of photosynthetic pigments,larger amount of releasing conchospores than the wild-type strain. Therefore,the improved strain PD-5 had great potential to be applied in commercial cultivation as a new strain.
- Pyropia dentata,
- blades,
- ⁶⁰Co-γray,
- pigmentation mutation,
- regenerated blades,
- improved strain

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References(36)

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