Diversity of mercuric reductase gene<em>merA</em> in Bohai Sea sediment

He Peiqing; Li Li; Liu Jihua; Fang Xisheng

doi:10.3969/j.issn.0253-4193.2015.08.010

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He Peiqing, Li Li, Liu Jihua, Fang Xisheng. Diversity of mercuric reductase genemerA in Bohai Sea sediment[J]. Haiyang Xuebao, 2015, 37(8): 105-116. doi: 10.3969/j.issn.0253-4193.2015.08.010

Citation:

He Peiqing, Li Li, Liu Jihua, Fang Xisheng. Diversity of mercuric reductase genemerA in Bohai Sea sediment[J]. Haiyang Xuebao, 2015, 37(8): 105-116. doi: 10.3969/j.issn.0253-4193.2015.08.010

He Peiqing, Li Li, Liu Jihua, Fang Xisheng. Diversity of mercuric reductase genemerA in Bohai Sea sediment[J]. Haiyang Xuebao, 2015, 37(8): 105-116. doi: 10.3969/j.issn.0253-4193.2015.08.010

Citation:

He Peiqing, Li Li, Liu Jihua, Fang Xisheng. Diversity of mercuric reductase genemerA in Bohai Sea sediment[J]. Haiyang Xuebao, 2015, 37(8): 105-116. doi: 10.3969/j.issn.0253-4193.2015.08.010

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Diversity of mercuric reductase genemerA in Bohai Sea sediment

doi: 10.3969/j.issn.0253-4193.2015.08.010

1.
Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
2.
Key laboratory of State Oceanic Administration for Marine Sedimentology & Environmental Geology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

Received Date: 2014-12-07
Rev Recd Date: 2015-06-02

Abstract

Abstract

Mercuric reductase gene merA had a potential role in mercuric reduction and detoxification in environment. During 2011-2012, surface sediments were collected from wastewater discharge of Dagu River in Tianjin Binhai New Area from Bohai Bay, near shore of Bohai Bay and Kenli aquaculture facility from Laizhou Bay. Clone libraries of merA from Actinomycete, Firmicutes, α-Proteobacteria and β/γ-Proteobacteria were constructed and the sequences were determined. Deduced MerA protein sequences of merA gene were then analyzed. The results showed that Bohai Sea sedimentary environment contained high diversity of MerA, and MerA diversity within Firmicutes, α-Proteobacteria and α/β/γ-Proteobacteria from wastewater discharge and aquaculture facility were higher than that in near shore of Bohai Bay. The MerA composition in different locations also differed, and within Firmicutes, the highest total abundance of MerAs that had the highest similarity with Bacillus sp. MB2021 was found in aquaculture facility(24.3%), while, the highest total abundance of MerAs that had the highest similarity with Paenisporosarcina sp. TG20 was found in wastewater discharge(46.3%). Different environment context, exogenous merA gene input, and co-selection caused by mercury and other heavy metal pollutants might together lead to the change of merA diversity, community composition in Bohai Sea sedimentary environment.
- Bohai Sea,
- wastewater discharge,
- aquaculture facility,
- mercuric reductase,
- diversity

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References(23)

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