<em>In situ</em> isolation of actinomycetes and screening bioactive potential from mangrove rhizosphere soils in Guangxi

Jiang Mingguo; Gan Guanghua; Yang Lifang; Li Xini; Yang Guiliu; Tuo Li; Sun Chenghang; Huang Ling; Lan Jinzhi

doi:10.3969/j.issn.0253-4193.2015.02.006

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Jiang Mingguo, Gan Guanghua, Yang Lifang, Li Xini, Yang Guiliu, Tuo Li, Sun Chenghang, Huang Ling, Lan Jinzhi. In situ isolation of actinomycetes and screening bioactive potential from mangrove rhizosphere soils in Guangxi[J]. Haiyang Xuebao, 2015, 37(2): 55-64. doi: 10.3969/j.issn.0253-4193.2015.02.006

Citation:

Jiang Mingguo, Gan Guanghua, Yang Lifang, Li Xini, Yang Guiliu, Tuo Li, Sun Chenghang, Huang Ling, Lan Jinzhi. In situ isolation of actinomycetes and screening bioactive potential from mangrove rhizosphere soils in Guangxi[J]. Haiyang Xuebao, 2015, 37(2): 55-64. doi: 10.3969/j.issn.0253-4193.2015.02.006

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In situ isolation of actinomycetes and screening bioactive potential from mangrove rhizosphere soils in Guangxi

doi: 10.3969/j.issn.0253-4193.2015.02.006

1.
Guangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Beihai 536000, China;School of Marine Sciences and Biotechnology, Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, Guangxi University for Nationalities, Guangxi Nanning 530008, China
2.
School of Chemistry and Chemical Engineering, Guangxi University for Nationalities Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Nanning 530008, China
3.
School of Marine Sciences and Biotechnology, Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, Guangxi University for Nationalities, Guangxi Nanning 530008, China
4.
Chinese Academy of Medical Sciences & Peking Union Medical College, Institute of Medicinal Biotechnology, Beijing 100050, China

Received Date: 2014-01-12
Rev Recd Date: 2014-05-02

Abstract

Abstract

In order to efficiently capture actinomycetes from mangrove rhizosphere soils in Guangxi, traps for particularly cultivating actinomycetes in situ were implanted into soil and were incubated for thirty days. A general selective isolation procedure was employed onto fifteen different media. Phylogenetic neighbour-joining tree was conducted on the 16S rRNA gene sequences of the isolates and type strains. The biological activities and enzyme production of the isolated strains were evaluated. One hundred thirty-three actinomycetes were recovered. Of the thirty-three randomly picked isolates, twenty were Streptomyces, eleven were Nocardiopsis and one was Actinosynnema. One isolate, GXUN10, was most closely related to Nocardiopsis with a low 16S rRNA gene sequence identity of 90.0%, and might represent a new genus. Seven, four, eighteen, six, ten and three isolates showed antimicrobial activities respectively to Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Proteusbacillus vulgaris, Hemolytic streptococcus and Pneumococcus. Fifty-five, sixty-two, twenty-two and seventy-two isolates respectively showed cellulase, amylase, collagen enzyme and esterase activities. This study provides further evidence that unusual and rare actinomycetes from mangrove rhizosphere soils in Guangxi can be recovered by in situ cultivation traps.
- in situ cultivation,
- mangrove,
- rhizosphere soils,
- actinomycetes,
- biological activity

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References(21)

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